热带作物学报2018，Vol.39Issue(2)：293-299,7.DOI:10.3969/j.issn.1000-2561.2018.02.013
龙眼胚性愈伤组织DlHOS1基因克隆与表达分析
Cloning and Expression Analysis of DlHOS1 in Embryogenic Callus of Dimocarpus longan Lour.
摘要
Abstract
To study the function of HOS1 in cold stress of Dimocarpus longan Lour.,the embryogenic callus of D.longan Lour were used to clone HOS1 gene (DlHOS1),and codon bias,bioinformatics and expression pattern of DlHOS1 were analyzed.The results showed that the full length sequence of DlHOS1 was 3 577 bp,containing an ORF 3 000 bp,encoding 999 amino acids,and the 5' UTR and 3' UTR was 162 bp and 415 bp respectively (GenBank accession number:KY990404).The codon bias level of DlHOS1 was low,and it biased toward the synonymous codons with A and T at the third codon position.Bioinformatics analysis indicated that DlHOS1 was an unstable and hydrophobic transmembrane protein,had no signal peptide and multilocated in cytoplasm and nucleus.The secondary structure was mainly composed of alpha helix and strand.Besides,DlHOS1 shared a very close relationship with citrus.qPCR analysis showed that DlHOS1 was response to the induction of low temperature and up-regulated at low temperature.The research suggested that DlHOS1 should be involved in cold resistance and low temperature stress in longan.关键词
龙眼/DlHOS1/基因克隆/密码子使用偏爱性/qPCRKey words
Dimocarpus longan Lour., DlHOS1/gene cloning/codon bias/Real-time fluorescent quantitative PCR分类
农业科技引用本文复制引用
肖小娥,徐小萍,李佳蜜,张梓浩,赖瑞联,林玉玲,赖钟雄..龙眼胚性愈伤组织DlHOS1基因克隆与表达分析[J].热带作物学报,2018,39(2):293-299,7.基金项目
国家自然科学基金(No.31572088、No.31672127) （No.31572088、No.31672127）
福建省重大科技专项(No.2015NZ0002-1). （No.2015NZ0002-1）
