热带作物学报2018，Vol.39Issue(2)：325-331,7.DOI:10.3969/j.issn.1000-2561.2018.02.017
绿僵菌Marho2基因功能初步分析
Functional Analysis of Marho2 in Metarhizium anisopliae
摘要
Abstract
Metarhizium anisopliaeis is widely used for biological control of pests in agriculture and forestry.The key steps of successfully infection are accompanied by the changes of cell morphology,which is the result of cell polarity establishment and cytoskeleton reorganization.Rho2,a member of Rho GTPases,plays an essential role in cell polarity establishment and normal morphogenesis.In order to illuminate the function of Marho2 related to the growth,development and pathogenic process in M.anisopliae,a Marho2 knockout vector was constructed by homologous recombination technique and transformed to Agrobacterium to obtain the Marho2 knockout strain (△Marho2).The results showed that compared with the wild-type strain,the deletion of Marho2 decreased the virulence of M.anisopliae,the amount of sporulation and the delay of spore germination,the deletion of Marho2 led to a significant reduction germination rate after heat-moisture treatment and UV-B.关键词
绿僵菌/Marho2/基因敲除/功能分析Key words
Metarhizium anisopliae/Marho2/gene knock-out/functional analysis分类
农业科技引用本文复制引用
陈丽,易萌,周江,孙云子,张传博..绿僵菌Marho2基因功能初步分析[J].热带作物学报,2018,39(2):325-331,7.基金项目
国家自然科学基金(No.31000881). （No.31000881）
