热带作物学报2018，Vol.39Issue(2)：332-337,6.DOI:10.3969/j.issn.1000-2561.2018.02.018
EDS1正调控茄子抗青枯病反应
Bacterial Wilt Resistance of EDS1 Positive Regulating Eggplant
摘要
Abstract
EDS1 (Enhanced Disease Susceptibility 1)plays a key role in plant disease resistance.To further analyze the EDS1 function in the eggplant bacterial wilt resistance,SmEDS1 was cloned according to the eggplant genome sequence.The bioinformation analysis showed that the max ORF box of SmEDS1 was 1 809 bp which coding 602 aa.SmEDS1 contained an LP and EP domain.After the resistant eggplant SmEDS1 gene was silenced by VIGS,the inoculated eggplant showed wilt at 7 days after inoculation with Ralstonia solanacearum.The result suggested that SmEDS1 positively regulated the eggplant bacterial wilt resistance.The gene expression of MAPK6,RAR1 increased while MAPK3,SIPK,PAD4,SGT1,TGA,EDR1,NPR1,ICS1,GLUA,EIL and HSP90 decreased after SmEDS1 was silenced.The expression of EBF2 and ACO5 were not regulated by SmEDS1.The results suggest that EDSI plays an important role in controling eggplant resistance to bacterial wilt.关键词
茄子/SmEDS1/青枯病Key words
eggplant/SmEDS1/bacterial wilt resistance分类
农业科技引用本文复制引用
李可,肖熙鸥,林文秋,李威,吕玲玲,曹必好..EDS1正调控茄子抗青枯病反应[J].热带作物学报,2018,39(2):332-337,6.基金项目
国家自然科学基金(No.31501774、No.31672156) （No.31501774、No.31672156）
海南省自然科学基金(No.20153120) （No.20153120）
中国热带农业科学院基本科研业务费专项资金(No.1630062017014). （No.1630062017014）
