热带作物学报2018，Vol.39Issue(3)：448-454,7.DOI:10.3969/j.issn.1000-2561.2018.03.007
土壤酸度对茶树根际土壤微生物群落多样性影响
Effect of Soil Acidity on Microbial Diversity in Rhizospheric Soils of Tea Plants
摘要
Abstract
To explore the effect of soil acidity on the microbial diversity in the rhizospheric soil of tea plants,the pH value and microbial diversity of the rhizospheric soils collected from'Tieguanyin'cultivar with different planted ages were determined.The results showed that the pH value of the rhizospheric soils decreased as the planted ages increased.There were 20 phospholipid fatty acid biomarkers (PLFAs),including 15 bacteria,2 fungi,2 actinomycetes and 1 protozoa,in the rhizospheric soils of tea plants.The amount of total PLFAs,and PLFA numbers of bacteria,actinomycetes,and protozoa were raised firstly and then declined,while the PLFA numbers of fungi showed an opposite trend.There were significant differences between different ages.The results were confirmed by the results of fluorescence quantitative PCR on numbers of bacteria,fungi and actinomycetes.Furthermore,PLFAs could be divided into two principle components,including PC1 and PC2,with contributing rates of 73.06％ and 25.51％,respectively.PC 1 was correlated significantly with bacteria,fungi,actinomycetes and protozoa,and PC2 correlated significantly with bacteria.The 11 PFLAs including bacteria,actinomycetes and protozoa were significantly and positively correlated with soil acidity,and 6 PFLAs including bacteria and fungi were significantly and negatively correlated with soil acidity.In brief,as the increase of planted ages,the acidity in the rhizospheric soils of tea tree declined and the soil microbial diversity significantly changed.This study provided theoretical support for the restoration of degraded tea gardens and the improvement of their acidified soils,thereby ensuring the tea yield and improving tea quality.关键词
茶树树龄/pH值/根际土壤/微生物多样性/磷脂脂肪酸Key words
tea plant age/pH value/rhizosphere soil/microbial diversity/PLFA分类
农业科技引用本文复制引用
王海斌,陈晓婷,丁力,邱丰艳,叶江华,贾小丽,孔祥海,何海斌..土壤酸度对茶树根际土壤微生物群落多样性影响[J].热带作物学报,2018,39(3):448-454,7.基金项目
国家重点研发计划(No.2016YFD0200900) （No.2016YFD0200900）
国家948项目(No.2014-Z36) （No.2014-Z36）
闽台作物特色种质创制与绿色栽培协同创新中心(No.2015-75) （No.2015-75）
中国博士后科学基金(No.2016M600493) （No.2016M600493）
福建省自然科学基金青年基金(No.2017J05057) （No.2017J05057）
福建省农业生态过程与安全监控重点实验室(福建农林大学)开放基金(No.NYST-2017-01) （福建农林大学）
福建省中青年教师教育科研项目(No.JAT170573) （No.JAT170573）
龙岩学院人才引进项目(No.LB2015001). （No.LB2015001）
