热带作物学报2018，Vol.39Issue(3)：513-519,7.DOI:10.3969/j.issn.1000-2561.2018.03.017
60Co-γ辐射对木薯生长的影响及其突变体的SCoT分析
Effects of Cassava Growth and SCoT Analysis on Mutant Under 60Co-rays Irradiation
摘要
Abstract
In order to discuss the effect on cassava growth and confirm DNA variations,Xinxuan 048 and South China 205 were radiated by 60Co-γ,and the dosage of radiation was 0 (CK),50,100,200,400,800 Gy,respectively.The cassava growth situation was obvered,then the phenotype identification was conducted and stable phenotypic variation was analyzed using SCoT molecular markers.The results showed that different cultivars had different sensibility to 60Co-γ.The half lethal dose of Xinxuan048 and South China 205 was 214 Gy,219 Gy,respectively,and the lethallty rate of the two cassava cultivars was 100％ when the dosage of gamma ray was 400.The cassava mutagenic dosage could be around 200 Gy,The phenotypic variability of Xinxuan 048 was 0％,0％,1.5％,5.4％,0％,0％ and Phenotypic variabilitys of South China 205 was 0％,0％,1.2％,3.5％,0％,0％,respectively under 0(CK),50,100,200,400,800 Gy.Eight mutants were obtained,and the types of the mutants were leaflet shape variation,petiole color variation,plant height variation,stem diameter variation.The results indicated differences between the parents and mutants could be distinguished with 1 lprimers on the molecular lever.The mutants are excellent materials for cassava breeding and mechanism study,and cassava mutants can be good distinguished by SCoT makers.The results could offer references for radiation mutation breeding in cassva.关键词
木薯/60Co-γ辐射/突变体/SCoT分析Key words
cassava/60Co-γ radiation/mutant/SCoT analysis分类
农业科技引用本文复制引用
谢向誉,尚小红,周慧文,陆柳英,曾文丹,曹升,赖大欣,严华兵..60Co-γ辐射对木薯生长的影响及其突变体的SCoT分析[J].热带作物学报,2018,39(3):513-519,7.基金项目
广西自然科学基金项目(No.2016GXNSFBA38010、No.2017GXNSFBA198223) （No.2016GXNSFBA38010、No.2017GXNSFBA198223）
