热带作物学报2018，Vol.39Issue(3)：520-524,5.DOI:10.3969/j.issn.1000-2561.2018.03.018
芦笋种质资源ISSR分析
ISSR Analysis of Asparagus officinalis L.Germplasm Resource
摘要
Abstract
An evaluation of the genetic diversity of 46 asparagus accessions was carried out based on inter-simple sequence repeat(ISSR)markers.The results showed that 21 ISSR primers produced 227 polymorphic bands across the 46 accessions.The values of polymorphic loci percentage,h and I was 82.01％,0.243 and 0.377,respectively.A cluster analysis was performed based on genetic similarity coefficients (GSCs) which ranged between 0.60 and 0.97 across the 46 accessions.The GSC results showed that abundant genetic diversity existed in the 46 accessions.There were 100 pairs germplasms with relatively strong heterosis.Based on the ISSR cluster analysis,the 46 asparagus accessions were clustered into Pacific Challenger 2,A and B Group A was consisted of Purple passion,Pacific purple and A1 which forming a compact group.And group B was comprised Jingpurple No.2,NJl189,Pacific 2000 and B1.Group A1 was from Walker Seed,Limseed,New Zealand,Canada and Jiangxi,whereas group B 1 Was from Jiangxi,Shandong,Beijing,Netherlands,America and Italy.The results would facilitate the optimal application of asparagus germplasm resources and provide an additional data for genetic improvement.关键词
芦笋/ISSR/遗传多样性/亲缘关系Key words
Asparagus officinalis L./ISSR/genetic diversity/genetic relationship分类
农业科技引用本文复制引用
陈河龙,高建明,张世清,郑金龙,黄兴,刘巧莲,易克贤..芦笋种质资源ISSR分析[J].热带作物学报,2018,39(3):520-524,5.基金项目
公益性行业(农业)科研专项经费项目(No.201003074) （农业）
海南省应用技术研发与示范推广专项(No.ZDXM2015047). （No.ZDXM2015047）
