热带作物学报2018，Vol.39Issue(5)：840-845,6.DOI:10.3969/j.issn.1000-2561.2018.05.002
秋水仙素诱导同源四倍体葛薯的研究
Induction of Autotetraploid Pueraria with Colchicine
摘要
Abstract
The morphological,agronomic,anatomical,photosynthetic and physiological indexes of the mutant strains of "Pueraria sp." seeds were analyzed using different concentrations of colchicine at different times.The results showed that the induction rate of tetraploid was the best with 0.15 g/L colchicine and 48 h treatment time.Compared with the diploid,the tetraploid Pueraria showed "greatness" in leaf thickness,stomatal size,pollen size,flower organ size and root tuber.The stem length,stem diameter,leaf length and leaf width,leaf weight,fruit weight,flower length,flower length,calyx vertical and horizontal diameter,vexilla length,ovary vertical and horizontal diameter and other indicators were significantly greater than diploid.The chlorophyll content,net photosynthetic rate,leaf soluble sugar content,soluble protein content,free proline (Pro) content,peroxidase activity (POD),superoxide dismutase (SOD) activity and catalase (CAT) of the tetraploid Pueraria sp were significantly higher than that of diploid and malondialdehyde(MDA) content decreased.It is proved that tetraploid potato is a very useful new germplasm,and it also points out a new direction for high yield and resistance breeding.关键词
葛薯/同源四倍体/诱导/光合特性/逆境生理指标Key words
pueraria/autotetraploid/induction/photosynthetic characteristics/stress physiological index分类
农业科技引用本文复制引用
陆建农,施玉珍,陈海标,罗茵茵,殷学贵..秋水仙素诱导同源四倍体葛薯的研究[J].热带作物学报,2018,39(5):840-845,6.基金项目
国家自然科学基金(No.31271759) （No.31271759）
广东省科技计划项目(No.2014A020208116). （No.2014A020208116）
