热带作物学报2018，Vol.39Issue(5)：852-857,6.DOI:10.3969/j.issn.1000-2561.2018.05.004
连作茶树根际土壤自毒潜力、酶活性及微生物群落功能多样性分析
Analysis on Autotoxic Potential, Enzyme Activity and Microbial Community Function Diversity of the Rhizosphere Soils from Tea Plants with Continuous Cropping Years
摘要
Abstract
To analyze effect of rhizosphere soil environment of tea tree to diversity of functional microbes,soils of 4-,9-and 30-planted years in Tieguanyin plantation were used as materials,and effects of planting age on soil autotoxicity,enzyme and divercity of functional microbes were explored.The result showed that rhizosphere soil inhibited root length of the lettuce(Lactuca sativa L.),cabbage(Brassica bara L.) and radish(Raphanus sativus L.) significantly with increased number of planted years.Activities of catalase,phosphatase,protease,sucrose enzyme,urease decreased significantly with increase in planted years,and activities of polyphenol oxidase and cellulose presented an opposite trend.The MDA levels increased significantly with increase of soil planted years.BIOLOG ECO ecological plate method results showed that soil microbes to carboxylic acids,phenolic acids,fatty acids,amino acid and amine carbon source utilization increased significantly with the increase of planting age,and sugar carbon source had no significant difference.Principal component analysis results showed that contribution rates of the principal component 1 (carboxylic acids,phenolic acids and fatty acid),the principal component 2(sugar carbon source) and the principal component 3(nitrogenous substances) reached 44.29％,42.24％ and 13.47％,respectively.These results showed that accumulation of acid chemicals led to soil acidification aggravating with the increase of planting age,and soil enzyme activity related to antioxidant and nutrient cycling declined,and so soil nutrient cycle suffocated.Furthermore,the number of good acid microbes increased,and soil microbial community structure and function simplified.In final,tea tree growth suffocated due to soil autotoxicity.关键词
茶树根际土壤/自毒作用/土壤酶活性/微生物功能多样性Key words
rhizosphere soil of tea tree/autotoxicity/activity of soil enzymes/microbial functional diversity分类
农业科技引用本文复制引用
王海斌,陈晓婷,丁力,邱丰艳,孔祥海,叶江华,贾小丽..连作茶树根际土壤自毒潜力、酶活性及微生物群落功能多样性分析[J].热带作物学报,2018,39(5):852-857,6.基金项目
国家重点研发计划(No.2016YFD0200900) （No.2016YFD0200900）
国家948项目(No.2014-Z36) （No.2014-Z36）
中国博士后科学基金(No.2016M600493) （No.2016M600493）
福建省自然科学基金青年基金(No.2017J05057) （No.2017J05057）
福建省农业生态过程与安全监控重点实验室(福建农林大学)开放基金(No.NYST-2017-01) （福建农林大学）
福建省中青年教师教育科研项目(No.JAT170573) （No.JAT170573）
龙岩学院人才引进项目(No.LB2015001). （No.LB2015001）
