热带作物学报2018，Vol.39Issue(5)：867-872,6.DOI:10.3969/j.issn.1000-2561.2018.05.006
甘蔗无根试管苗光合自养生根过程中叶片生理生化特征的变化
Changes of Physiological and Biochemical Characteristics in Leaves During Photoautotrophic Rooting of in vitro Sugarcane Plantlets
摘要
Abstract
Photoautotrophic rooting technique is newly developed for in vitro sugarcane plantlets,which has a significant advantage over the conventional heterotrophic rooting technique.The purpose of this study is to reveal the physiological and biochemical mechanism of the new rooting technique,and ultimately to provide a theoretical basis for improving the technology.In this study,the sugarcane cultivar GT44 was used as the explant,and the in vitro sugarcane plantlets were transplanted in the mixed sandy soil substrate for photoautotrophic rooting in the sunlight greenhouse after sprayed with ABT 2 rooting powder solution and hardened for 24 h.During the rooting process,samples were taken continuously to investigate the rooting rate and growth status,the total chlorophyll,carbohydrates (sucrose,soluble sugars),three types of endogenous hormones (IAA,ABA,CTK) contents and peroxidase (POD) activity in the leaves were detected.The results showed that the plantlets were stressed and got yellow slightly in the leaves due to environmental changes,then turned green.Seven days later the older leaves turned yellow and aging,but more new leaves appeared.The first signed adventitious roots broke through the epidermis at 5 d and 100％ of plantlets completed their regeneration of adventitious roots before 9 d.The chlorophyll content decreased firstly then increased and decreased,but jumped up after 9 d.The sucrose and soluble sugar contents at first steadily increased then drastically declined from day 7 to day 9 and later jumped up.The ABA content and POD activity increased at first and then decreased.The contents of IAA and CTK were declined at first and then increased,which was in opposite to that of ABA.The findings of the study suggested that photoautotrophic rooting of sugarcane in vitro plantlets was divided into three phases:the first was the adaptation phase from 0 d to 7 d,the second was the heterotrophic-autotrophic switching phase from 7 d to 9 d,and the third was the autotrophic function recovery phase from 9 d to 15 d.Successful recovery of the autotrophic function was essential for high survival of sugarcane plantlets.关键词
甘蔗/试管苗/光合自养生根/叶片/生理生化Key words
sugarcane/in vitro plantlet/photoautotrophic rooting/leaf/physiology and biochemistry分类
农业科技引用本文复制引用
刘丽敏,何为中,刘红坚,余坤兴,范业赓,翁梦苓..甘蔗无根试管苗光合自养生根过程中叶片生理生化特征的变化[J].热带作物学报,2018,39(5):867-872,6.基金项目
广西农业科学院科技成果转化项目(农成转2015001) （农成转2015001）
国家星火计划项目(No.2015GA790007) （No.2015GA790007）
广西农业科学院科技发展基金项目(No.2015JZ04) （No.2015JZ04）
广西创新驱动发展专项资金项目(桂科AA17202042-11). （桂科AA17202042-11）
