热带作物学报2018，Vol.39Issue(5)：906-912,7.DOI:10.3969/j.issn.1000-2561.2018.05.012
2个14-3-3蛋白基因在香蕉果实成熟过程中的表达分析
Expression Analysis of Two 14-3-3 Genes During Ripening of Banana Fruit
摘要
Abstract
Two cDNAs homologous with 14-3-3 protein genes were obtained by screening cDNA library of banana fruit by degenerate primer,landing PCR and RACE (rapid amplification of cDNA ends) approaches,and they were designated as Ma14-3-3d and Ma14-3-3h,respectively.Multiple alignment and homology comparison of deduced amino acid sequences of Ma14-3-3d and Ma14-3-3h together with Ma14-3-3a,Ma14-3-3c,Ma14-3-3e,and Ma14-3-3i revealed that they shared high similarity and specificity.Phylogenetic analysis showed that Ma14-3-3d with Ma14-3-3a,Ma14-3-3c,Ma14-3-3e,and Ma14-3-3i belonged to the non-ε group 14-3-3 genes,while Ma14-3-3h was classed into the ε group.Expression analysis showed that Ma14-3-3d and Ma14-3-3h were differentially expressed in different organs of banana as determined by RT-PCR (reverse transcriptase-polymerase chain reaction),Ma14-3-3d was expressed in roots,stems,leaves,flowers and fruits of banana,and its expression in stems,leaves and flowers was higher than that in roots and fruits;the expression of Ma14-3-3h in roots,flowers and fruits was higher,and its expression in stems and leaves was low.Expression of Ma14-3-3d was obviously induced by ethylene as determined by qRT-PCR analysis,while the expression of Ma14-3-3h in ethylene treated and 1-MCP treated fruits was very low,its correlation with fruit ripening was not obvious.It is speculated that Ma14-3-3d may be closely related to the physiological process of ripening of banana fruit,and may be involved in ethylene biosynthesis,regulation of fruit ripening and signal transduction.关键词
香蕉/Ma14-3-3d/Ma14-3-3h/成熟/表达分析Key words
banana/Ma14-3-3d/Ma14-3-3h/ripening/expression analysis分类
农业科技引用本文复制引用
李美英,金志强,杨小亮,贾彩虹,夏启玉,郭静远,贺萍萍,徐碧玉..2个14-3-3蛋白基因在香蕉果实成熟过程中的表达分析[J].热带作物学报,2018,39(5):906-912,7.基金项目
现代农业产业技术体系建设专项资金(No.CARS-31) （No.CARS-31）
国家自然科学基金(No.31201663). （No.31201663）
