热带作物学报2018，Vol.39Issue(5)：913-919,7.DOI:10.3969/j.issn.1000-2561.2018.05.013
龙眼Mn-SOD基因的表达及其启动子功能分析
Expression of Mn-SOD and Functional Analysis of Its Promoter in Dimocarpus longan Lour.
摘要
Abstract
Expression of Mn-SOD was analyzed in embryogenic callus (EC) under abiotic stress and exogenous hormone treatment in Dimocarpus longan,and its promoter activities were studied in a tobacco transient expression system.Results showed that the expression of Mn-SOD was inhibited in longan EC treated by red and blue lights;Mn-SOD firstly increased and then decreased in white light treated-EC,and it has contrary expression pattern under green light treatment.The expression of Mn-SOD was inhibited under salt stress,and was not affected by sucrose and days treatment.In a certain range of concentration,exogenous hormones IAA,GA3,MeJA,SA and ETH could induce or inhibit the expression of Mn-SOD in longan EC,suggesting that longan Mn-SOD was involved in the response of exogenous hormones.Four deletions of longan Mn-SOD promoter fused to the GUS reporter gene were constructed and transient transformed into tobacco.The results showed that the activities of the four deletions of longan Mn-SOD promoter were lower than that of CaMV35S promoter,but all of them had the ability to activate the GUS gene.GUS activity and quantitative expression analysis both showed that 3'terminal deletion of MSD-pro4 (-420 bp) promoter is the strongest,followed by the terminal 5'deletions of MSD-pro1(-669～-1 bp),and the weakest were MSD-pro2 (-432 ～-1 bp) and MSD-pro3 (-267～-1 bp),suggesting that longan Mn-SOD promoter within the-669～-432bp region contains important positive regulatory elements,while the -1～432 region contains a negative regulatory element.关键词
龙眼/Mn-SOD/启动子/瞬时表达Key words
Dimocarpus longan/Mn-SOD/promoter/transient expression分类
生物科学引用本文复制引用
陈晓慧,曾丽兰,徐小萍,王亚婷,张梓浩,陈裕坤,林玉玲,赖钟雄..龙眼Mn-SOD基因的表达及其启动子功能分析[J].热带作物学报,2018,39(5):913-919,7.基金项目
福建省重大专项(No.2015NZ0002-1) （No.2015NZ0002-1）
国家自然科学基金(No.31572088、No.31672127). （No.31572088、No.31672127）
