热带作物学报2018，Vol.39Issue(5)：920-925,6.DOI:10.3969/j.issn.1000-2561.2018.05.014
紫参薯查尔酮合成酶及异构酶基因的克隆与表达分析
Clonging and Expression Analysis of DaCHS and DaCHI Gene in Dioscorea alata
摘要
Abstract
Chalcone synthase (CHS) and Chalcone isomerase (CHI) play an important role in the process of flavonoids synthesis.To investigate the function of the DaCHS and DaCHI gene in anthocyanin biosynthesis pathway of Dioscorea alata.L.,the complete genes of chalcone synthase(CHS) and chalcone isomerase(CHI) were isolated from the turber of D.alata,by reverse transcription-polymerase chain reaction (RT-PCR) named as DaCHS and DaCHI.Sequencing results showed that the cDNA sequence of DaCHS gene contained an open reading frame (ORF) of 825 bp encoding a protenin of 274 amino acids,DaCHI gene contained an open reading frame (ORF) of 663 bp encoding a protenin of 221 amino acids.Phylogeneticanalyses showed that DaCHS and DaCHI had high identity to CHS and CHI protein of many plants.The highly similarity of DaCHS protenin was EgCHS protenin with 90％ amino identity.The highly similarity of DaCHS protenin was CaCHI protenin with 67％ amino identity.Quantitative real-time PCR analysis showed that the DaCHS and DaCHI expression had tissue specificity.The relative expression level of flowers were the highest in DaCHS.In DaCHI,the tubers had the hightest relative expression level.That expression analysis in different development periods of D.alata.tuber,DaCHS and DaCHI all had the hightest relative expression in expansion period of tubers.The cloning and analysis of full-length cDNA sequences of DaCHS and DaCHI genes should lay a foundation for the genetic regulation of anthocyanin biosynthesis pathway and the mechanism of anthocyanin formation.关键词
花青素/查尔酮合成酶/查尔酮合成异构酶/基因克隆/表达分析Key words
anthocyanin/chalcone synthase/chalcone isomerase/gene cloning/expression analysis分类
农业科技引用本文复制引用
赵景梅,黄东益,张青,许云..紫参薯查尔酮合成酶及异构酶基因的克隆与表达分析[J].热带作物学报,2018,39(5):920-925,6.基金项目
海南省重点研发计划项目(No.ZDYF2016205) （No.ZDYF2016205）
国家自然科学基金(No.31260346). （No.31260346）
