热带作物学报2018，Vol.39Issue(5)：926-930,5.DOI:10.3969/j.issn.1000-2561.2018.05.015
墨兰(Cymbiduim sinense)组培快繁技术体系研究
Micropropagation in vitro of Cymbidium sinensis
摘要
Abstract
The propagation of C.sinense is greatly restricted by the low seed germination rate due to immature embryo and no endosperm under natural conditions.In this study,the rhizomes of well grown and uniformity induced by seeds in C.sinense were used as the materials.The influence of different plant growth regulators on culture in vitro of C.sinense was studied.The results showed that the optimum basic medium for the multiplication and growth of the rhizomes was MS medium,and the best hormone combinations of multiplication was 6-BA 1.0 mg/L+IBA 0.1 mg/L,with a multiplication rate of rhizomes up to 10.33.The optimum plant growth regulator combination for differentiation of rhizomes was 6-BA 5.0 mg/L+ NAA 0.5 mg/L,with a regeneration rate 6.43.All buds produced roots on basic medium with no plant hormone on MS medium.The present study established an efficient propagation system during industrial seedling production and provided a good theoretical and technological support for improving seedlings quality of C.sinense and its industrial production in a large scale.关键词
墨兰/组织培养/根状茎/分化Key words
Cymbidium sinensis/tissue culture/rhizome/differentiation分类
农业科技引用本文复制引用
许申平,袁秀云,王默霏,崔波..墨兰(Cymbiduim sinense)组培快繁技术体系研究[J].热带作物学报,2018,39(5):926-930,5.基金项目
郑州师范学院科技创新团队支持计划. （）
