热带作物学报2018，Vol.39Issue(5)：931-939,9.DOI:10.3969/j.issn.1000-2561.2018.05.016
杉木天冬酰胺合成酶基因的克隆与表达分析
Cloning and Expression Analysis of Asparagine Synthetase Gene from Cunninghamia lanceolata
摘要
Abstract
In order to investigate the underlying mechanism of ASN gene of Cunninghamia lanceolata in the growth stages and under abiotic stresses,RT-PCR combined with RACE was employed to clone the complete eDNA sequence and DNA sequence from Cunninghamia lanceolata seedling,and the expression of ClASN1 gene and the Ash content under various growth stages and abiotic stresses were analyzed.The results showed that the complete cDNA sequence of ClASN1 was 1 609 bp,encoding 443 amino acids.The bioinformatics software predicted that ClASN1 protein was unstable and hydrophilic with transmembrane structure without signal peptide,located in peroxidase and with various phosphorylation sites.Anglicizing phylogenetic tree of ASN genes in different plants indicated that ClASN1 had the closest relative with Picea sitchensis and Pinus sylvestris.The full length of genomic ClASN1 was 3 210 bp,which contained 10 exons and 9 introns,qPCR results showed that the expression of ClASN1 increased with the time elapsed from seed germination to seedling stage,and was also induced under drought stress,aluminum stress and light dark stress,moreover,the general expression tendencies of ClASN1gene accorded with the content of Asn under various stages from seed germination to seedling growth and different stresses expect for aluminum stress 24 h and light stress 24 h.In conclusion,the above results suggested that ClASN1 may play a role in the various stages from seed germination to seedling growth and stress responses.关键词
杉木/ASN基因/克隆/表达分析Key words
Cunninghamia lanceolata/ASN gene/cloning/expression analysis分类
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饶丽莎,官清娜,林思祖,叶义全,许珊珊..杉木天冬酰胺合成酶基因的克隆与表达分析[J].热带作物学报,2018,39(5):931-939,9.基金项目
中央财政支持地方专项(No.6213c0111). （No.6213c0111）
