山东医药2018，Vol.58Issue(11)：1-4,4.DOI:10.3969/j.issn.1002-266X.2018.11.001
KU55933对化疗后胶质母细胞瘤细胞株U87增殖、凋亡的影响及其机制探讨
Effects of KU55933 on proliferation and apoptosis of glioblastoma cells U87 after chemotherapy
摘要
Abstract
Objective To explore the influence and mechanism of inhibition of ATM/ATR(KU55933) on the proliferation and apoptosis of glioblastoma cells U87 treated with chemotherapy.Methods U87 cells were randomly divided into three groups:KU55933 group,TMZ group,and control group (without intervention).In the KU55933 group,10 μmol/L KU55933 was added,and after 1 h,50 mg/mL temozolomide (TMZ) was added;in the TMZ group,50 mg/mL TMZ was added.The cells were cultured in the 37 ℃ and 5％C02 incubator.Then we compared the OD values,apoptosis rates,and relative protein expression of cell cycle checkpoint kinase 1 (Chk1),phosphorylation Chk1 (pChk1),cell cycle checkpoint kinase 2 (Chk2),phosphorylation Chk2 (pChk2),Cyclin B,Cyclin D1,and Cleave-Caspase-3.Results The TMZ group and the KU55933 group had significantly lower OD values at 24,48,and 72 h as compared with the control group (all P ＜ 0.05),and the KU55933 group had significantly lower OD values at 24,48,and 72 h as compared with the TMZ group (all P ＜ 0.05).The TMZ group and the KU55933 group had significantly higher apoptosis rates as compared with the control group (all P ＜ 0.05),and the KU55933 group had a significantly higher apoptosis rate as compared with the TMZ group (P ＜ 0.05).The relative protein expression levels of pChk1,pChk2,and Cleaav-Caspase-3 in the TMZ group and the KU55933 group were significantly higher than those in the control group (all P ＜ 0.05),and the relative protein expression levels of Cyclin B and Cyclin D1 in the TMZ group and the KU55933 group were significantly higher than those in the control group (both P ＜ 0.05);compared with the TMZ group,the KU55933 group had significantly lower relative protein expression of pChk1,pChk2,Cyclin B,Cyclin D1 and a higher relative protein expression of Cleaav-Caspase-3.Conclusion KU55933 may significantly inhibit the proliferation and accelerate the apoptosis of U87 cells after chemotherapy by down-regulating the expression of pChk protein,Cyclin B protein,Cyclin D1 protein,and reversing the cell cycle arrest.关键词
ATM/ATR阻滞剂/替莫唑胺/胶质母细胞瘤Key words
inhibition of ATM/ATR/temozolomide/glioblastoma分类
医药卫生引用本文复制引用
王倩,蔡炜嵩..KU55933对化疗后胶质母细胞瘤细胞株U87增殖、凋亡的影响及其机制探讨[J].山东医药,2018,58(11):1-4,4.基金项目
辽宁省科学技术计划项目(201602812). （201602812）
