摘要
Abstract
Objective To observe the effects of different doses of gypenosides (Gyp) on the migration and cell morphology of human glioblastoma U87 cells in vitro and to explore the mechanism.Methods U87 cells in the logarithnic growth phase were added with 100 μL of 10 and 5 mg/mL Gyp solution and the same volume of PBS (referred as groups A,B,and C),and then given the routine cultivation.The cell morphology,migration ability,and interleukin-6 protein levels were compared in each group.Results In the group A,the cells grew very slowly with small volume,the pyknosis was very obvious and the phenomenon of cell attachment disappeared nearly.In the group B,the cells grew slowly with reduced volume and cytoplasm pyknosis,some cells even ruptured and died,and the cell attachment decreased.In the group C,the cell growth was regular with clear outline and full-shape,the cell body was translucent,and the adherent growth was good.The area of cell migration in the groups A,B,and C accounted for 54.8％,82.4％,and 98.6％ of the original scratch area (all P＜0.05);the number of transmembrane cells in the groups A,B,and C accounted for 19.8％,58.9％,and 100％ (all P ＜ 0.05);the protein levels of IL-6 in the groups A,B,and C were high,moderate,and low,respectively;significant differences were found between every two groups (all P ＜ 0.05).Conclusion The Gyp inhibits the growth and metastasis ability of U87 cells with the best effect at 10 mg/mL Gyp,and the mechanism may be associated with the inhibition of IL-6 protein in U87 cells.关键词
绞股蓝总皂苷/胶质母细胞瘤/U87细胞/细胞形态/细胞迁移能力/IL-6蛋白Key words
gypenosides/glioblastoma/U87 cells/cell morphology/cell migration/interleukin 6 protein分类
医药卫生
