中国畜牧杂志2018，Vol.54Issue(6)：65-68,74,5.DOI:10.19556/j.0258-7033.2018-06-065
萝卜硫素对体外成熟小鼠卵母细胞谷胱甘肽合成相关基因表达的影响
Effects of Sulforaphane on the Expression of Glutathione Related Gene in vitro Maturation of Mouse Oocytes
摘要
Abstract
By comparing the maturation of oocytes in vivo and in Vitro in synthesizing glutathione related genes,and trying to use Sulforaphane to increase GSH synthesis in oocytes.Three weeks mouse were used in this experiments,and found that in the natural mature oocytes Gclc,Gclm near MⅡ was higher,and the cumulus cells near MⅠ was higher.The expression of Gclc and Gclm in vitro maturation oocytes was significantly lower than that in vitro in both MⅠand MⅡ (P＜0.05),while the expression of MⅠ was lower than that in vivo.There was no difference in Gss.After that,we attempted to add 1,5,10,20 μmol/L Sulforaphane to improve the content of GSH in oocytes.The results showed that adding 10 μmol/L SFN can significantly improve the mRNA level of GSH related genes (P＜0.05),and eventually improved the GSH content of the oocytes in vitro in order to achieve the purpose of optimizing the system of in vitro maturation.关键词
体外成熟/卵母细胞/谷胱甘肽/萝卜硫素Key words
in vitro maturation/Oocytes/Glutathione/Sulforaphane分类
农业科技引用本文复制引用
王文娟,郭思淼,安磊..萝卜硫素对体外成熟小鼠卵母细胞谷胱甘肽合成相关基因表达的影响[J].中国畜牧杂志,2018,54(6):65-68,74,5.基金项目
北京市生猪产业创新团队(BAIC02-2018) （BAIC02-2018）
国家重点研发项目(2017YFD0501901) （2017YFD0501901）
