安徽医科大学学报2018，Vol.53Issue(1)：58-62,5.DOI:10.19405/j.cnki.issn1000-1492.2018.01.013
局灶性脑缺血再灌注损伤对小鼠脑组织平滑肌细胞α-SMA表达的影响
Effects of focal cerebral ischemia-reperfusion injury on α-SMA expression in mouse brain smooth muscle cells
摘要
Abstract
Objective To investigate the effect of focal cerebral ischemia-reperfusion injury on the expression of a-smooth muscle actin (α-SMA) in mouse brain smooth muscle cells. Methods The middle cerebral artery occlusion (MCAO model) was established by right cerebral embolism ischemia in the C57BL/6 mouse model. The expressions of a-SMA in the smooth muscle cells of the cerebral ischemia reperfusion mice cerebral ischemia 2 h reperfusion 24 h group (the experimental group) and the sham operation group and the blank control group were observed by qRT-PCR, Western blot and immunohistochemistry. Results TTC (2, 3, 5-triphenyltetrazolium chloride )staining showed that the cerebral infarct size of the experimental group was significantly higher than that of the sham operation group and the blank control group. The experimental group had obvious cerebral edema. Western blot and qRT-PCR showed that the expression of a-SMA in focal cerebral ischemia-reperfusion group was significantly higher than that in sham operation group and blank control group at 2 h after reperfusion for 24 h (P ＜ 0. 05). Conclusion Focal cerebral ischemia-reperfusion injury can stimulate the brain smooth muscle cells expression of a-SMA. The expression of a-SMA in brain is significantly higher than that in sham operation group and blank control group at 2 h after reperfusion.关键词
小鼠/MCAO模型/缺血再灌注损伤/α-平滑肌肌动蛋白/平滑肌细胞Key words
mouse/MCAO model/ischemia-reperfusion injury/α-smooth muscle actin/smooth muscle cells分类
医药卫生引用本文复制引用
陈二峰,谈启松,马春春,卞尔保,赵兵..局灶性脑缺血再灌注损伤对小鼠脑组织平滑肌细胞α-SMA表达的影响[J].安徽医科大学学报,2018,53(1):58-62,5.基金项目
安徽省自然科学基金(编号:1608085MH225) （编号:1608085MH225）
