安徽医科大学学报2018，Vol.53Issue(1)：71-75,5.DOI:10.19405/j.cnki.issn1000-1492.2018.01.016
膀胱癌中miR-203的表达及其对吉西他滨化疗敏感性的影响
Expression of miR-203 in bladder carcinoma and its sensitivity to gemcitabine chemotherapy
摘要
Abstract
Objective To investigate the expression of microRNA-203 (miR-203) in bladder cancer and its effect on chemosensitivity of gemcitabine. Methods The sensitivity of the bladder cancer cell line to gemcitabine was analyzed by Vita-Blue assay and the half-inhibitory concentration (IC50 ) was measured. The expression level of miR-203 was detected in 27 cases of bladder cancer tissues and adjacent tissues by Taqman-qRT-PCR. All the time, the expression levels of miR-203 were also detected in bladder cancer cell lines 5637, Um-Uc-3, J82, SCaBER, T24, Biu87. Construction of miR-203 overexpression vector (pINDUCER21-EGFP-miR-203) with a tetracycline-induced expression system, which was transfected into gemcitabine-resistant Um-Uc-3 cells and was induced with doxycycline(No doxycycline was negative control). Simultaneously, miR-203 Antagomir and Antagomir control were transfected into T24 cells respectively. The sensitivity to gemcitabine and expression levels of miR-203 were also detected using Vita-Blue and Taqman-qRT-PCR in T24 and Um-Uc-3. Results The expression of miR-203 was significantly lower in bladder cancer tissues than in adjacent tissues. The expression level of miR-203 was the highest in bladder cancer cell line T24 and the lowest in Um-Uc-3 cell line (P ＜0. 01). Bladder cancer cell line T24 and Um-Uc-3 to IC50 values of gemcitabine chemotherapy were (0. 46 ±0. 08) ng/ml and (5. 94 ±0. 53) ng/ ml, respectively (P ＜0. 01);pINDUCER21-EGFP-miR-203 (D + ) can remarkably enhance its chemosensitivity in Um-Uc-3, whereas miR-203 Antagomir can also significantly suppress the chemosensitivity of T24 cells to gemcitabine. Conclusion miR-203 is associated with the development and progression of bladder cancer, and its high expression level may improve the chemosensitivity of bladder cancer to gemcitabine.关键词
膀胱癌/miR-203/吉西他滨/化疗敏感性Key words
bladder cancer/miR-203/gemcitabine/chemosensitivity分类
医药卫生引用本文复制引用
林垚,沈洲,牛三强,许言,刘治,宣强..膀胱癌中miR-203的表达及其对吉西他滨化疗敏感性的影响[J].安徽医科大学学报,2018,53(1):71-75,5.基金项目
安徽省自然科学基金(编号:1708085QH179) （编号:1708085QH179）
