安徽医科大学学报2018，Vol.53Issue(2)：171-175,5.DOI:10.19405/j.cnki.issn1000-1492.2018.02.002
二甲双胍丁酸盐对人肺腺癌A549细胞增殖、凋亡与迁移能力的影响
Effects of metformin butyrate on the proliferation, apoptosis and mobility of the A549 of the human lung adenocarcinoma
摘要
Abstract
Objective To study the effects of different concentrations of metforminbutyrate (MFB) on the proliferation, apoptosis and mobility of the human lung adenocarcinoma (A549 ) after different time of intervention. Methods A549 cells cultered in vitro were treated with MFB at a gradient concentration (0, 0.5, 1, 2, 4, 8 mmol/ L), and after 24 h and 48 h, the MFBs inhibition rate on the A549 cell proliferation was detected by MTT. The apoptosis rate of A549 cells was analyzed by using a fluid cell instrument after 24 h and 48 h. The effect of MFB on the mobility of A549 cell lines was analyzed with scratch test. Results The MFB experiment showeded that MFB had a inhibitory effect on the proliferation of 549 cells with a dose and time dependent manner (P < 0. 05). The experimental results showed that the apoptosis rate of the experimental group was greater than that of the control group(P < 0. 05), which had a certain time and concentration dependence (P < 0. 05). The experimental results of the scratches showed that the experimental group cell migration rate was less than the control group, high concentration treatment group cell migration rate was less than the low concentration treatment group (P < 0. 05), and drug action after 48 h cell migration rate was less than 24 h (P < 0. 05 ). Conclusion MFB can inhibit the proliferation and migration of human pulmonary adenocarcinoma A549 cells in vitro and promote apoptosis, and its intensity may be related to its concentration and time.关键词
二甲双胍丁酸盐/肺腺癌/增殖/凋亡/迁移Key words
metformin butyrate/lung adenocarcinoma/proliferation/apoptosis/migration分类
医药卫生引用本文复制引用
陈晴晴,束军,谢庆书,沈继龙..二甲双胍丁酸盐对人肺腺癌A549细胞增殖、凋亡与迁移能力的影响[J].安徽医科大学学报,2018,53(2):171-175,5.基金项目
安徽省自然科学基金面上项目(编号:1308085MH141) （编号:1308085MH141）
安徽高校省级自然科学研究项目(编号:KJ2011A173) （编号:KJ2011A173）
