安徽医科大学学报2018，Vol.53Issue(3)：331-338,8.DOI:10.19405/j.cnki.issn1000-1492.2018.03.001
人表皮生长因子受体2与wnt/β-catenin信号相互作用促进卵巢癌细胞转移
Human epidermal growth factor receptor 2 interacts with wnt/β-catenin signaling to promote ovarian cancer cell metastasis
摘要
Abstract
Objective To investigate the interaction between human epidermal growth factor receptor 2 (HER2) and wnt/β-catenin signaling in ovarian cancer cells and its effect on ovarian cancer cell metastasis. Methods Western blot was used to detect the expression of HER2 and T cell factor 4 (TCF4) in ovarian cancer cell line SK-OV3. Lentivirus vector was used to respectively construct the expression of HER2 and TCF4 knocked down in SK-OV3 cell. Transwell and would healing assay was used to compare the migration ability of cells before and after knockdown, and the effects of receptor stimulator EGF and wnt3a and small molecule inhibitor TCF4 targeting TCF4/β-catenin were observed respectively, the effects of HER2, β-catenin and TCF4 on the expression and cell migration were observed as well. Results Ovarian cancer cell line SKOV3 overexpresses HER2 and TCF4 (P＜0.05). The protein level of HER2 was decreased (P＜0.05), and the cell migration ability was significantly decreased (P＜0.05), and the protein level of β-catenin and TCF4 was decreased (P＜0.05). After EGF was added, the expression of HER2 in SKOV3 cells increased with the prolongation of stimulation time, and the expression of β-catenin and TCF4 was also enhanced (P＜0.05). After adding wnt3a, the expression of β-catenin and TCF4 increased with the duration of stimulation, and the expression of HER2 was higher (P＜0.05). After adding iCRT14, the expression of β-catenin and TCF4 decreased with the duration of the stimulus, and the expression of HER2 decreased(P＜0.05), The cell migration ability of SKOV3-shHER2 and SKOV3-shTCF4 cells was significantly increased after adding EGF and wnt3a (P＜0.05). SKOV3 cells were treated with iCRT14, and the cell migration was significantly decreased (P＜0.05). Conclusion The interaction of HER2 and wnt/β-catenin signaling in ovarian cancer cells plays a common role in promoting the metastasis of ovarian cancer cells.关键词
卵巢癌/HER2/wnt/TCF4Key words
ovarian cancer/HER2/wnt/TCF4分类
医药卫生引用本文复制引用
刘芬芬,高倩,程民,徐婷娟,任荟蓉,沈国栋..人表皮生长因子受体2与wnt/β-catenin信号相互作用促进卵巢癌细胞转移[J].安徽医科大学学报,2018,53(3):331-338,8.基金项目
安徽省自然科学基金面上项目(编号:1408085MH167) （编号:1408085MH167）
安徽省科技攻关项目(编号:1301042094) （编号:1301042094）
