安徽医科大学学报2018，Vol.53Issue(3)：343-347,5.DOI:10.19405/j.cnki.issn1000-1492.2018.03.003
白花丹醌对CIA大鼠滑膜细胞增殖及TNF-α、IL-1β和MMP-3表达的影响
Effect of plumbagin on proliferation and the expressions of TNF-α, IL-1β and MMP-3 in CIA-FLS in rat
摘要
Abstract
Objective To observe the effects of plumbagin on proliferation and the expressions of tumor necrosis factor-a(TNF-α), interleukin-1β (IL-1β) and matrix metalloproteinase-3 (MMP-3) in fibroblast-like synoviocytes(FLS) induced by lipopolysaccharide(LPS). Methods Using type II collagen and Freund's complete adjuvant to make arthritis model in rat. CIA-FLS were primary cultured in vitro, the effect of drug on proliferation of CIA-FLS was detected by MTT method. CIA-FLS were divided into CIA control group, LPS control group, methotrexate group, three concentration groups of plumbagin 1.0, 1.5 or 2. 0 μmol/L; after being incubated with each drug for 48 hours, the levels of TNF-a, IL-1β and MMP-3 in cell culture supernatant were assayed by ELISA; the protein content of MMP-3 in CIA-FLS was mesured by Western blot. Results The result of MTT showed that plumbagin could inhibit the proliferation of CIA-FLS in different degrees, presenting in a concentration and time dependence manner. Compared with LPS control group, the contents of TNF-α, IL-1β and MMP-3 in cell culture supernatant were significantly decreased in methotrexate, the 1.0, 1.5 and 2. 0 μmol/L of plumbagin groups (P＜ 0. 001); methotrexate or plumbagin could also significantly reduce the protein expression of MMP-3 in CIA-FLS (P＜ 0. 001). Conclusion Plumbagin can inhibit the proliferation of CIA-FLS and the inflammatory reaction induced by LPS via decreasing the expressions of TNF-α, IL-1β and MMP-3.关键词
白花丹醌/Ⅱ型胶原蛋白诱导的关节炎/滑膜细胞/肿瘤坏死因子-α/白介素-1β/基质金属蛋白酶-3/脂多糖Key words
plumbagin/CIA/FLS/TNF-a/IL-1β/MMP-3/LPS分类
医药卫生引用本文复制引用
钟毓娟,李丽,李勇文,杨成芳,方舒萍,程琪..白花丹醌对CIA大鼠滑膜细胞增殖及TNF-α、IL-1β和MMP-3表达的影响[J].安徽医科大学学报,2018,53(3):343-347,5.基金项目
广西高校科学技术研究项目(编号:KY2015LX279) （编号:KY2015LX279）
