安徽医科大学学报2018，Vol.53Issue(3)：390-395,6.DOI:10.19405/j.cnki.issn1000-1492.2018.03.013
佛手柑内酯抑制p62/NF-κB信号轴增强顺铂对肝癌杀伤的影响
Bergapten strengthens the anti-tumor activity of cisplatin by suppressing p62/NF-κB pathway in hepatocellular carcinoma
摘要
Abstract
Objective To investigate the effect of bergapten strengthening the anti-tumor activity of cisplatin in hepatocellular carcinoma in vitro. Methods The hepatocellular carcinoma cells (HepG2 and Bel-7402) were treated with 1 mol/L bergapten and/or 1 mol/L cisplatin, and the apoptosis ratio was detected by Hoechst33258 staining and Annexin V-FITC/PI analysis. The expression of anti-apoptosis related genes were detected by real-time quantitive polymerase chain reaction. The activity of NF-kB transcription factors were detected by luciferase reporter gene analysis. The expression of p65 and p62 were detected by Western blot. Results Compared with cisplatin only treatment, the ratio of apoptosis nucleus and cells was remarkable increased by combination of bergapten and cisplatin both in HepG2 and Bel-7402 (P＜ 0. 05 for each). The mRNA level of BCL2, BCL2L1, XIAP and BIRC5 was decreased by combination treatment with bergapten and cisplatin. The transcript ability of NF-kB and the protein level of p65 and p62 was decreased by combination treatment with bergapten and cisplatin. Conclusion Bergapten could strengthen the anti-tumor activity of cisplatin by suppressing p62/NF-κB pathway in hepatocellular carcinoma in vitro.关键词
佛手柑内酯/肝癌/顺铂/p62/NF-KB信号轴Key words
bergapten/hepatocellular carcinoma/cisplatin/p62/NF-KB pathway分类
医药卫生引用本文复制引用
郑志明,邹海鹏,卢敏娟,林碧华,周克元..佛手柑内酯抑制p62/NF-κB信号轴增强顺铂对肝癌杀伤的影响[J].安徽医科大学学报,2018,53(3):390-395,6.基金项目
国家自然科学基金(编号:81272434) （编号:81272434）
广东省医学科学技术研究基金(编号:A2016395 ) （编号:A2016395 ）
广东省中医药局科研项目(编号:20172085) （编号:20172085）
中山市科技计划(编号:2015B1061) （编号:2015B1061）
