安徽医科大学学报2018，Vol.53Issue(3)：428-432,5.DOI:10.19405/j.cnki.issn1000-1492.2018.03.021
脑胶质瘤患者肿瘤组织和外周血HOPX基因甲基化的检测
Detection of HOPX gene methylation in tumor tissue and plasma from glioma patients
摘要
Abstract
Objective To detect the methylation status of homeodomain only protein X (HOPX) gene in tumor tissue and peripheral blood of glioma and explore its clinical significance. Methods Fifty-two cases of glioma tissue and peripheral blood plasma samples from patients with glioma were selected as the experimental group. Twenty cases of necrotic brain tissue and peripheral blood samples from patients with craniocerebral injury were selected as control group. Methylation specific polymerase chain reaction and bisulfate sequencing PCR were used to detect the methylation status of HOPX gene in the experimental group and control group. Results The rate of HOPX gene methylation in the glioma tissue and necrotic brain tissue was 71. 15％ (37/52) and 20. 0％ (4/20) respectively and the rate of HOPX methylation gene in peripheral blood of glioma patients and craniocerebral injury patients was 65. 38％ (34/52) and 15. 0％ (3/20) respectively. The rate of HOPX gene methylation in tumor tissue and peripheral blood of glioma patients was not significantly correlated with gender, age, pathological type, tumor location and pathological grade. Conclusion The rate of HOPX gene methylation in tumor tissue and peripheral blood of glioma patients is high, which may be related to the occurrence of glioma. Because of its high sensitivity, it is expected to provide clues and basis for early diagnosis and provide new ideas for the treatment of glioma patients.关键词
脑胶质瘤/甲基化/HOPX基因Key words
glioma/methylation/HOPX gene分类
医药卫生引用本文复制引用
侯保森,徐培坤,王斌,李显雄,李式浩,曹明..脑胶质瘤患者肿瘤组织和外周血HOPX基因甲基化的检测[J].安徽医科大学学报,2018,53(3):428-432,5.基金项目
安徽省科技攻关计划项目(编号:1301042208) （编号:1301042208）
