安徽医科大学学报2018，Vol.53Issue(6)：856-860,5.DOI:10.19405/j.cnki.issn1000-1492.2018.06.007
芹菜素改善肥胖大鼠肾脏氧化应激损伤
Apigenin improves oxidative stress damage in obese rats
摘要
Abstract
Objective To investigate the effects of apigenin on renal metabolic status and its possible mechanism in obese rats. Methods All 4-week-oldmale SD rats were randomly divided into Con group and HFD group. The HFD group was fed with high-fat diet for 12 weeks, then divided into Apigenin group and Obesity group randomly. The Apigenin group wastreated with apigenin 40 mg/( kg·d) for another 4 weeks. The Con and Obesity groups of rats were received 0.9% normal saline in the meantime. Western blot and immunohistochemical were used for pro-tein expression assays. Results ① After 4 weeks apigenin treatment, the rat weights were decreased compared with Obesity group(t=1.80,P=0.096), but the difference was not statistically significant. ②DHE staining was used to evaluate the O2- formation in the kidney. In apigenin group, the content of O2- was decreased distinctly compared with Obesity(t=2.79,P=0.049). ③ The protein expression of p-P67 and p-p65 were decreased com-pared with Obesity (P＜0.05) after apigenin treatment. ④ LC3BII/LC3BI and Beclin1 protein levels in kidney were increased significantly compared with the other two groups after apigenin treatment(P＜0.05). Conclusion Apigenin alleviates the obese rats renal oxidative stress and inflammation state, which may be related to the activa-tion of autophagy pathway.关键词
芹菜素/肥胖/自噬/氧化应激/炎症Key words
apigenin/obesity/autophagy/oxidative stress/inflammation分类
医药卫生引用本文复制引用
黄斌,陶小根,陈剑,陈超,叶山东..芹菜素改善肥胖大鼠肾脏氧化应激损伤[J].安徽医科大学学报,2018,53(6):856-860,5.基金项目
安徽省自然科学基金面上项目(编号:11040606M159) （编号:11040606M159）
