安徽医科大学学报2018，Vol.53Issue(6)：885-889,5.DOI:10.19405/j.cnki.issn1000-1492.2018.06.012
PLA激活JNK信号通路促进NCI-H292细胞凋亡
PLA activates JNK signal pathway to promote apoptosis of NCI-H292 cells
摘要
Abstract
Objective To investigate the signaling pathway and molecular mechanism of PLA-induced NCI-H292 apoptosis. Methods The concentration gradients with PLA were divided into five groups: 0, 10, 20, 40 and 60 mg/L. After NCI-H292 cell treatment for 24 hours, Western blot was employed to detect the phosphorylation level of JNK in each group. The set groups included the control group, PLA group (40 mg/L), SP (JNK inhibitor) group, and PLA+SP group. After 24 hours’ treatment, flow cytometer was used to detect NCI-H292 apoptotic rate in each group, while Western blot was adopted to detect their Bcl-2/Bax, Caspase-3, P-JNK/JNK and β-actin pro-tein expression levels in NCI-H292 cells. Results The NCI-H292 apoptotic rates in the black control group, PLA group, SP group, and PLA+SP group were (5.13 ± 1.07 ) % , (22.62 ± 1.66 ) % , (5.69 ± 0.14 ) % ,(8.99 ± 3.73)% , respectively. According to the detection results by Western blot, the BCL-2/Bax ratio in PLA-induced NCI-H292 cells was reduced ( P＜0.01) , the expression of Caspase-3 was increased ( P＜0.001) . In PLA-in-duced NCI-H292 cells, the phosphorylation level of JNK was enhanced (P＜0.001), and SP600125 inhibited the JNK phosphorylation induced by PLA ( P＜0.001) . Conclusion PLA may lead to the increase of NCI-H292 ap-optosis level by inducing JNK signaling pathway.关键词
哮喘/c-Jun氨基末端激酶/细胞凋亡Key words
asthma/c-jun amino terminal kinase/cell apoptosis分类
医药卫生引用本文复制引用
吴莎莎,范晓云,梁雅雪,徐玉菲,王亚妮,管明龙..PLA激活JNK信号通路促进NCI-H292细胞凋亡[J].安徽医科大学学报,2018,53(6):885-889,5.基金项目
安徽省自然科学基金(编号:1708085MH 178) （编号:1708085MH 178）
2016年高校学科(专业)拔尖人才学术资助重点项目(编号:gxbjZD2016036) （专业）
