安徽医科大学学报2018，Vol.53Issue(6)：918-922,5.DOI:10.19405/j.cnki.issn1000-1492.2018.06.019
维生素D3对博来霉素诱发小鼠肺纤维化的影响
Effects of vitamin D3 on bleomycin-induced pulmonary fibrosis in mice
摘要
Abstract
Objective To investigate the effects of vitamin D3 on bleomycin ( BLM)-induced pulmonary fibrosis in mice. Methods 96 adult male C57BL/6J mice (8 weeks, 24～26 g) were randomly divided into eight groups:Control group, vitamin D3 (VitD3) group, BLM 1 d, 7 d and 21 d groups, and VitD3+BLM 1 d, 7 d and 21 d groups. In bleomycin group, mice were intratracheally injected with bleomycin ( 3 mg/kg) . In VitD3 + BLM group, mice were intraperitoneally injected with 1,25(OH)2D3(1 μg/kg) daily, beginning at 30 min before BLM injection. VitD3 mice were injected with 1,25(OH)2D3(1 μg/kg) daily. Control mice were injected with saline daily. Mice were euthanized at 1 d, 7 d, and 21 d after BLM, respectively. Some lungs were collected for real-time RT-PCR. Some lungs were excised for histopathologic examination and immunohistochemistry. Results As expected, BLM-induced damage of alveolar structure and pulmonary fibrosis in mice. Interestingly, 1,25(OH)2D3 attenuated BLM-induced damage of alveolar structure and pulmonary fibrosis in mice. Further study found that BLM up-regulated oxidant enzyme gene expression and down-regulated antioxidant enzyme gene expression. 1, 25 ( OH)2D3inhibited BLM-induced up-regulation of oxidant enzyme gene and down-regulation of antioxidant enzyme gene. Moreover, strong 3-nitrotyrosine immunoreactivity was observed in the lungs 1 d, 7 d and 21 d after BLM in-jection, and 1,25(OH)2D3significantly attenuated BLM-induced protein nitration in the lungs. Conclusion Vita-min D3 may reduce BLM-induced pulmonary tissue destruction and interstitial fibrosis through anti-pulmonary oxi-dative stress.关键词
博来霉素/维生素D3/肺纤维化/氧化应激Key words
bleomycin/vitamin D3/pulmonary fibrosis/oxidative stress分类
医药卫生引用本文复制引用
王熹,赵卉,徐德祥,陈远华..维生素D3对博来霉素诱发小鼠肺纤维化的影响[J].安徽医科大学学报,2018,53(6):918-922,5.基金项目
安徽省自然科学基金(编号:1508085MH192) （编号:1508085MH192）
