安徽医科大学学报2018，Vol.53Issue(7)：1010-1016,7.DOI:10.19405/j.cnki.issn1000-1492.2018.07.005
miR-133b通过靶向调控EGFR影响食管鳞癌的侵袭、转移
miR-133b regulates esophageal squamous cell carcinoma invasion and metastasis by targeting EGFR
摘要
Abstract
Objective To investigate the role and mechanism of miR-133b in cell invasion and metastasis of esoph-ageal squamous cell carcinoma( ESCC) . Methods Bioinformatics tools were used to predict the potential target gene of miR-133b; dual luciferase reporter gene assay was conducted to verify the prediction. Cell proliferation, wound healing and transwell assay were performed to investigate the effects of miR-133b on cell proliferation, mi-gration and invasion in esophageal squamous cell carcinoma. qRT-PCR was conducted in a cohort of 33 pairs of ES-CC tissues and correspondingly adjacent tissues, then the relationship between the miR-133b and clinical pathologi-cal features weer analysed. Results Bioinformatics tools predicted that EGFR was the potential target gene of miR-133b, dual luciferase reporter gene assay verified that miR-133b could bind to a specific sequence of the 3′-UTR of the mRNA of EGFR. Overexpression of miR-133b in ESCC cell could inhibit proliferation(ECA109: P ＜0. 05, KYSE150: P＜0. 01), migration(ECA109: P＜0. 01, KYSE150: P＜0. 01) and invasion(ECA109:P＜0. 01, KYSE150: P＜0. 01) in vitro. The results demonstrated that EGFR, the target of miR-133b in ESCC, which up-regulated in ESCC tissues was negatively related to miR-133b(P＜0. 01), and miR-133b was frequently downregu-lated in esophageal squamous cell carcinoma tissues and its decrement correlated with TNM stage of ESCC and lym-phatic metastasis (P both＜0. 05). Conclusion MiR-133b may inhibit esophageal carcinoma proliferation, inva-sion and metastasis by targeting EGFR, it plays a negative role in the progression of ESCC.关键词
食管鳞癌/miR-133b/EGFR/侵袭/转移Key words
esophageal squamous cell carcinoma/miR-133b/EGFR/invasion/metastasis分类
医药卫生引用本文复制引用
曾薇,朱金峰,孔德华,单莉..miR-133b通过靶向调控EGFR影响食管鳞癌的侵袭、转移[J].安徽医科大学学报,2018,53(7):1010-1016,7.基金项目
国家自然科学基金地区基金项目(编号:81660397) （编号:81660397）
