安徽医科大学学报2018，Vol.53Issue(7)：1021-1025,5.DOI:10.19405/j.cnki.issn1000-1492.2018.07.007
肝细胞生长因子在原发性开角型青光眼视神经损伤中的作用及机制研究
The role of hepatocyte growth factor in optic nerve injury of primary open-angle glaucoma and its mechanism
摘要
Abstract
Objective To examine whether c-met-receptor-activation by hepatocyte growth factor ( HGF) can pro-mote retinal ganglion cell survival and regeneration and the possible mechanism of neuroprotective effects by HGF in a chronic glaucoma rat model. Methods SD rats were randomly divided into three groups:normal control group, chronic intraocular hypertension group and chronic intraocular hypertension HGF-injection group. Chronic glaucoma model was made in Sprague Dawley rats by cauterizing three episcleral veins; animals were examined for changes in intraocular pressure by a iCare tonometer. HGF was injected immediately at a dosage of 4 μg intravitreally after cauterizing episcleral veins. The levels of HGF in anterior chamber were measured by ELISA. The cellular distribu-tion of c-met in the retina were examined by anti-c-met and immunostaining. Apoptosis of retinal ganglion cells were visualized by terminal deoxynucleotidyl transferase dUTP nick end labeling( TUNEL) staining. Changes in the expression pattern of Akt, p-Akt, Bcl-2 and Bcl-xl were determined by Western blot. Results Early postoperative IOP increased significantly, and maintained for 2 weeks. Compared with the normal group, the levels of HGF in the anterior chamber of rats with high intraocular pressure was significantly increased. Compared with the chronic in-traocular hypertension group, the TUNEL-labeled RGC in HGF-injection group was significantly decreased, the difference was statistically significant(P＜0. 05). In the retina, c-met was expressed mainly in cells in the ganglion cell. In retinal samples, compared with the normal control group, p-Akt, Bcl-2 and Bcl-xl expressions were signifi-cantly downregulated in ocular hypertensive eyes(P＜0. 05), while upregulated in the HGF-treated animals com-pared with high intraocular pressure rats(P＜0. 05). Conclusion HGF may play a protective role in chronic ocu-lar hypertension by activating the Akt signaling pathway and increasing its downstream antiapoptotic protein expres-sion.关键词
肝细胞生长因子/青光眼/视网膜神经节细胞/Akt/凋亡Key words
hepatocyte growth factor/glaucoma/retinal ganglion cells/Akt/apoptosis分类
医药卫生引用本文复制引用
刘超,陶黎明,梁坤,蒋正轩,张康玉,刘贺婷..肝细胞生长因子在原发性开角型青光眼视神经损伤中的作用及机制研究[J].安徽医科大学学报,2018,53(7):1021-1025,5.基金项目
安徽高校自然科学研究项目(编号:KJ2016A340) （编号:KJ2016A340）
2017年度高校优秀青年人才支持计划项目(编号:gxyqZD2017033) （编号:gxyqZD2017033）
