安徽医科大学学报2018，Vol.53Issue(7)：1026-1032,7.DOI:10.19405/j.cnki.issn1000-1492.2018.07.008
芍药苷减轻糖尿病小鼠肾组织炎症与JAK2/STAT3信号通路的关系
Effect of paeoniflorin on preventing inflammation in diabetic mice kidneys and its relationship with JAK2/STAT3 signaling pathway
摘要
Abstract
Objective To investigate the protective effect and possible mechanism of paeoniflorin ( PF) on inflam-mation in diabetic mice kidneys. Methods 60 male C57BL/6J mice were randomly divided into five groups: nor-mal control group, diabetic group, PF 25 mg/kg group, PF 50 mg/kg group and PF 100 mg/kg group. At 12 weeks, blood glucose, kidney weight/ body weight, 24 h urinary albumin excretion rate ( UAER) were measured. Kidney pathological lesions and damage grade were detected by light microscopy. The expression of CD68, p-JAK2 and p-STAT3 were detected by immunohistochemistry. Western blot was used to detect the expression of p-JAK2 and p-STAT3 while tumor necrotic factor-α (TNF-α), interleukin-1β (IL-1β), monocyte chemoattractant protein-1 (MCP-1) and inducible nitric oxide synthase (iNOS) mRNA level was evaluated by qRT-PCR. Results In week 12, compared with the control group, the levels of blood glucose, kidney weight/body weight, UAER level and kidney pathological lesions grade were higher in diabetic group ( P＜0. 05, P＜0. 01) , while kidney weight/body weight, UAER level and kidney pathological lesions grade decreased significantly after PF intervention ( P＜0. 05, P＜0. 01). CD68, p-JAK2, p-STAT3 protein expression and TNF-α, IL1-β, MCP-1, iNOS mRNA expres-sion were higher in diabetic group ( P＜0. 01) and lower in PF 25, 50, 100 mg/kg intervention groups ( P＜0. 05, P＜0. 01) . Conclusion PF significantly improves diabetic nephropathy progression and these protective effects might associate with the inhibition of JAK2/STAT3 signaling pathway and inflammation.关键词
糖尿病肾脏疾病/芍药苷/JAK2/STAT3信号通路/炎症/巨噬细胞Key words
diabetic kidney disease/paeoniflorin/JAK2/STAT3 signaling pathway/inflammation/macrophage分类
医药卫生引用本文复制引用
李新玉,邵云侠,王坤,吴永贵..芍药苷减轻糖尿病小鼠肾组织炎症与JAK2/STAT3信号通路的关系[J].安徽医科大学学报,2018,53(7):1026-1032,7.基金项目
国家自然科学基金(编号:81374034) （编号:81374034）
