安徽医科大学学报2018，Vol.53Issue(7)：1032-1036,5.DOI:10.19405/j.cnki.issn1000-1492.2018.07.009
利拉鲁肽对肥胖相关性肾病的作用以及与自噬通路的关系
Effect of liraglutide on obesity-related glomerulopathy and its relationship with autophagy pathway
摘要
Abstract
Objective To evaluate the effect of the glucagon-like peptide 1 ( GLP-1) receptor agonist liraglutide on obesity-related glomerulopathy ( ORG) in mice and its relationship with autophagy pathway SIRT-1/AMPK. Meth-ods Thirty-four male mice were randomly divided into normal control group(n=12) and hyperlipidemia group (n=22). The high-fat group was randomly divided into GLP-1 receptor agonist group (liraglutide group) and high-fat control group. After intervention for 12 weeks, renal pathological staining, serological and urinalysis were per-formed. Western blot was used to detect the expression of autophagy pathway protein in kidney tissues. Data were analyzed by means of homogeneity of variance. Results Compared with the normal control group, obesity group mice glomerular hypertrophy, mesangial matrix increased significantly, and accompanied by focal stage sclerosis. Compared with obesity group, liraglutide group mice kidney lesions significantly reduced. Compared with the normal control group, body weight, wet weight of right kidney and urine microalbumin and blood lipids in obesity group were significantly increased ( P＜0. 05) . Compared with obesity group, the above indicators of liraglutide group were significantly decreased (P＜0. 05). Compared with the normal control group, the obesity group SIRT-1, P-AMPK and LC3B were significantly decreased, P62 cumulative increased. Compared with obesity group, liraglutide group SIRT-1, P-AMPK and LC3B were significantly increased, P62 cumulative decreased. Conclusion Liraglu-tide can reduce urine microalbumin and improve the degree of glomerular hypertrophy, focal stage sclerosis and me-sangial matrix in high fat-induced obesity-related nephropathy mice, and the mechanism underlying may contribute to induction of autophagy in ORG mice.关键词
肥胖相关性肾病/利拉鲁肽/自噬Key words
obesity-related glomerulopathy/liraglutide/autophagy分类
医药卫生引用本文复制引用
李娜,郭晶晶,霍琴琴,潘天荣,钟兴..利拉鲁肽对肥胖相关性肾病的作用以及与自噬通路的关系[J].安徽医科大学学报,2018,53(7):1032-1036,5.基金项目
安徽高校自然科学研究项目(编号: KJ2018A0202、KJ2017A174) （编号: KJ2018A0202、KJ2017A174）
安徽省科技厅公益性技术应用研究联动项目(编号:1501ld04042) （编号:1501ld04042）
