安徽医科大学学报2018，Vol.53Issue(7)：1057-1062,6.DOI:10.19405/j.cnki.issn1000-1492.2018.07.014
miR-30b-5p抑制胆固醇合成和结直肠癌细胞增殖
miR-30b-5p suppresses cholesterol synthesis and colorectal cancer cell proliferation
摘要
Abstract
Objective To investigate the role and involved mechanisms of miR-30b-5p in colorectal cancer. Meth-ods RNA was isolated from the colorectal cancer and adjacent tissues, the serum of patients and healthy people. miR-30b-5p expression was determined by qRT-PCR assay. miR-30b-5p inhibitors were transfected into HT-29 cells. miR-30b-5p mimics were transfected into HCT-116 cells. Cell viability was measured by CCK-8 assay. Chol-esteryl Ester Quantitation Kit was used to detect the cholesterol level in indicated cells. qRT-PCR was performed to determine LDLR expression. Western blot was used to detect the protein abundance of LDLR. LDLR was further knocked down using siRNA assay in HT-29 cells transfected with miR-30b-5p inhibitors. LDLR expression, choles-terol level and cell proliferation were detected. Results miR-30b-5p level was decreased in the tumor tissues and serum of colorectal cancer patients. It was also down-regulated in Caco-2, HT-29 and HCT-116 colorectal cancer cells compared with NCM460 cells. miR-30b-5p inhibitors led to enhanced the proliferation, cholesterol abundance and LDLR expression of HT-29 cells. miR-30b-5p mimics suppressed the viability, cholesterol level and LDLR ex-pression of HCT-116 cells. In addition, knockdown of LDLR decreased the cholesterol abundance and suppressed the viability of HT-29 cells transfected with miR-30b-5p inhibitors. Conclusion miR-30b-5p exerts as a tumor suppressive microRNA in colorectal cancers. It inhibits the proliferation of colorectal cancer cells through inhibiting LDLR expression and cholesterol synthesis.关键词
结直肠癌/miR-30b-5p/LDLR/胆固醇/增殖Key words
colorectal cancer/miR-30b-5p/LDLR/cholesterol/proliferation分类
医药卫生引用本文复制引用
张亚珍,何贵省,吴晓明,宋杰峰,吴煌福..miR-30b-5p抑制胆固醇合成和结直肠癌细胞增殖[J].安徽医科大学学报,2018,53(7):1057-1062,6.基金项目
海南省卫生厅科研立项课题(编号:琼卫2011-73) （编号:琼卫2011-73）
海南省科学技术厅重点研发计划(编号:ZDYF2017087) （编号:ZDYF2017087）
