北华大学学报（自然科学版）2018，Vol.19Issue(4)：465-468,4.DOI:10.11713/j.issn.1009-4822.2018.04.010
羟基红花黄色素A抗心肌缺血-再灌注损伤作用与钙超载相关性研究
Correlation between Effect of HSYA on Anti-myocardial Ischemia Reperfusion and Calcium Overload
摘要
Abstract
Objective To explore the relationship between effect of HSYA on myocardial ischemia-reperfusion injury and calcium overload by establishing myocardial ischemia-reperfusion injury rat model through surgical method. Method Myocardial cell hypoxia reperfusion damage model of the cultured SD rats was employed to conduct the experiment,which was divided into five groups:the control group ( Sham,1) ,ischemia-reperfusion group for 1 hour (1 h,2),ischemia-reperfusion group for 24 hours (24 h,3),ischemia-reperfusion group for 1 hour treated with HSYA(1 h+,4),ischemia-reperfusion group for 24 hours treated with HSYA (24 h+,5). Changes of calcium ion concentration in the cardiomyocytes of ischemia-reperfusion rats were compared,and the expression of myocardial cell membrane ATPase MCA and SERCA were detected. Results Compared with the normal control group ( Sham ) , intracellular calcium ion concentration increased significantly in ischemia- reperfusion group (1 h,24 h),and the phenomenon was more obvious for 24 h reperfusion (P<0. 05). Compared with the reperfusion group (1 h,24 h),calcium ion concentration decreased significantly in HSYA group after treatment (1 h +,24 h +)(P<0. 05). The expressions of sarcoplasmic reticulum calcium ATPase SERCA in 1 + h and 24 h+groups decreased significantly compared with the Sham group(P<0. 05),but they significantly increased compared with 1 h and 24 h groups ( P<0. 01 ). Conclusion Myocardial ischemia-reperfusion injury was associated with calcium overload,and calcium overload was caused by myocardial ischemia-reperfusion resulting from the effect of HSYA which was regulated by SERCA,rather than PMCA.关键词
羟基红花黄色素A/心肌缺血-再灌注/钙超载/细胞膜钙ATP酶Key words
hydroxy safflower yellow A/myocardial ischemia-reperfusion/calcium overload/cell membrane calcium ATPase分类
医药卫生引用本文复制引用
王丽娜,梅玉,苗艳芳,杜小玉,孙玉芹..羟基红花黄色素A抗心肌缺血-再灌注损伤作用与钙超载相关性研究[J].北华大学学报（自然科学版）,2018,19(4):465-468,4.基金项目
吉林省教育厅科学技术研究项目(2010123). （2010123）
