北京大学学报（医学版）2018，Vol.50Issue(1)：117-122,6.DOI:10.3969/j.issn.1671-167X.2018.01.020
选择性激光熔化种植体对早期骨矿化沉积率的影响
Influence of implants prepared by selective laser melting on early bone healing
摘要
Abstract
Objective: To evaluate the influence of the rough surface of dental implants prepared by selective laser melting (SLM) on early bone healing around titanium implants. Methods: A total of sixteen titanium implants were involved in our research,of which eight implants were prepared by SLM (TIXOS (R) Cylindrical, Leader-Novaxa, Milan, Italy; 3. 3 mm × 10 mm, internal hex) and the other eight were sandblasted, large-grit and acid-etched (SLA) implants (IMPLUS (R) Cylindrical,Leader-Novaxa,Milan, Italy; 3. 3 mm x 10 mm, internal hex). All of the dental implants were inserted into the healed extraction sockets of the mandible of two adult male Beagle dogs. Half of the dental implants were designed to be healed beneath the mucosa and the other half were intended to be healed transgingivally and were immediately loaded by acrylic resin bridge restoration. Three types of tetracycline fluorescent labels, namely calcein blue, alizarin complexone and calcein, were administered into the veins of the Beagle dogs 2,4, and 8 weeks after implant placement respectively for fluorescent evaluation of newly formed bone peri-implant. Both Beagle dogs were euthanized 12 weeks after implant insertion and the mandible block specimens containing the titanium implants and surrounding bone and soft tissue of each dog were carefully sectioned and dissected. A total of 16 hard tissue slices were obtained and stained with toluidine blue for microscopic examination and histomorphometric measurements. Histological observation was made for each slice under light microscope and laser scanning confocal microscope (LSCM). Comparison on new bone formation around titanium implants of each group was made and mineral apposition rate (MAR) was calculated for each group. Results: Dental implants prepared by selective laser melting had achieved satisfying osseointegration to surrounding bone tissue after the healing period of 12 weeks. Newly formed bone tissue was observed creeping on the highly porous surface of the SLM implant and growing into the pores of surface structure. Higher MAR values were shown for SLM implants compared with SLA implants (P <0. 01 ). Conclusion: Dental implants prepared by selective laser melting could promote early bone healing and improve mineral apposition rate.关键词
牙种植体/选择性激光熔化/骨生成/矿化沉积率/荧光染料Key words
Dental implants/Selective laser melting (SLM)/Osteogenesis/Mineral apposition rate (MAR)/Fluorescent dyes分类
医药卫生引用本文复制引用
刘婧寅,陈飞,葛严军,魏菱,潘韶霞,冯海兰..选择性激光熔化种植体对早期骨矿化沉积率的影响[J].北京大学学报（医学版）,2018,50(1):117-122,6.基金项目
国家自然科学基金(81400484)和北京大学口腔医院青年科研基金项目(Ys 020213)资助 （81400484）
Supported by the National Natural Science Foundation of China (81400484) and the Youth Foundation of Peking University School and Hospital of Stomatology (Ys 020213) （81400484）
