北京大学学报（医学版）2018，Vol.50Issue(1)：176-182,7.DOI:10.3969/j.issn.1671-167X.2018.01.030
RGD多肽修饰多孔钽对MG63成骨样细胞-钽界面形态及成骨因子表达的影响
Effects of RGD peptides-grafted porous tantalum on morphological change of MG63 osteoblasts-tantalum conjunctive interface and expression of osteogenesis factors
摘要
Abstract
Objective: To investigate the effects of the Arg-Gly-Asp polypeptedes (RGD) peptides-modified porous tantalum surface on osteoblasts morphology and expressions of osteogenesis factors,and to evaluate RGD peptides promotes junctura ossium of tantalum-bone interface in vivo. Methods: RGD peptides of different concentrations (1 g/L, 5 g/L, and 10 g/L) were loaded to porous tantalum slices with a diameter of 10 mm and a thickness of 3 mm by physical absorption. The 3rd generation of MG63 cells were co-cultured with tantalum and divided into 4 groups: Ta-cells (control) group,1 g/L cells/ Ta/RGD group, 5 g/L cells/Ta/RGD group, and 10 g/L cells/Ta/RGD group. Porous tantalum composites and osteoblasts-tantalum interface were observed by scanning electron microscopy. The adhesion rate of osteoblasts was detected and immunocytochemistry was used to detect the expressions of filamentous actin (F-actin),osteocalcin (OC ) and fibronectin (FN). Results: The scanning electron microscope (SEM) revealed that osteoblasts distributed on the surface of porous tantalum and secreted extracellular matrix on outside and inner of micro-pores. The osteoblasts adhesion rate on porous tantalum modified with RGD was higher than that in the unmodified porous tantalum at the end of 24,48, and 72 hours. The best adhesion effect was got in 5 g/L cells/Ta/RGD group at hour 48 [(68. 07 ± 3. 80) vs. (23.40 ±4. 39),P <0. 05]. The results of immunocytochemistry showed that the expressions intensity of F-actin,OC and FN in osteoblasts on porous tantalum modified groups with RGD were stronger than that in the unmodified groups,and the expressions of 5 g/L cells/Ta/RGD group were significantly higher than those in the 10 g/L group and 1 g/L group [OC: (18.08 ±0.08) vs. (15. 14 ±0. 19),P<0.05; (18.08 ±0. 08) vs. (14.04 ±0.61),P <0.05. FN: (24.60 ±0.98) vs. (15. 90 ±0. 53),P< 0.05; (24.60 ±0. 98) vs. (15. 30 ± 0. 42),P<0. 05. F-actin: (29.20 ±1.31) vs. (24.50 ± 1.51),P <0.05; (29.20 ± 1.31) vs. (16.92 ±0.40),P <0.05]. Correspondingly F-actin in osteoblasts was showed in longitudinal arrangement, and the expressions intensity was stronger than those OC and FN. Conclusion: The RGD peptides is beneficial to enhance adhesion of osteoblast, spreading and reorganization of cytoskeleton on porous tantalum surface and improve the interface morphology,further promoting osteoblasts-tantalum conjunctive interface osseointegration.关键词
RGD多肽/钽/细胞黏附/成骨细胞Key words
Arg-Gly-Asp polypeptedes peptides/Tantalum/Cell adhesion/Osteoblasts分类
医药卫生引用本文复制引用
甘洪全,王茜,张辉,刘鑫,邓华民,宋会平,王志强,李琪佳..RGD多肽修饰多孔钽对MG63成骨样细胞-钽界面形态及成骨因子表达的影响[J].北京大学学报（医学版）,2018,50(1):176-182,7.基金项目
“十二五”国家科技支撑计划项目(2012BAE06B03)、河北省科技支撑项目(16277776D)、河北省卫生和计划生育委员会2015年临床医学优秀人才培养项目(361036)、河北省医学科学研究重点课题计划(20160225)资助 （2012BAE06B03）
Supported by the National Ministry of Science and Technology Support Project for "12th Five-Year Plan" (2012BAE06B03),Science and Technology Support Project of Hebei Province (16277776D), Health and Family Planning Commission Clinical Medical Project of Hebei Province (361036), Key Propram for Medical Science Project of Hebei Province (20160225) （2012BAE06B03）
