川北医学院学报2018，Vol.33Issue(3)：338-342,5.DOI:10.3969/j.issn.1005-3697.2018.03.011
铜绿假单胞菌外膜蛋白F与HSV-1VP22融合DNA疫苗的构建与表达
Construction and expression of fusion vaccine DNA of Pseudomonas aeruginosa outer membrane protein F and HSV-1VP22
摘要
Abstract
Objective:To construct the DNA vaccine for the outer membrane protein F from Pseudomonas aerufinosa and VP22 from herpes simplex virus 1 fusion gene, and to study its express in eukaryotic cells. Methods: The eukaryotic expression vectors pVAX1-OprF、pVAX1-VP22、pVAX1-VP22-OprF and pVAX1-OprF-VP22 were constructed. The VP22 carboxy-terminal was expressed in prokaryotic cells and purified by electroelution. Polyclonal antibody was developed by immunizing BALB/c mice with purified recom-binant protein. The specificity of BALB/c mice antiserum was measured by Western blot. The recombinant plasmids were transfected in-to eukaryotic cells by LipofectamineTM. The expressions of recombinant eukaryotic vectors were investigated by Western blot. Results:The polyclonal antibody against VP22 protein was successfully prepared. The recombinant vectors could be expression in eukaryotic cells. Conclusion:The eukaryotic expression vector for the outer membrane protein F from Pseudomonas aerufinosa and VP22 from her-pes simplex virus 1 fusion gene can express in eukaryotic cells,which may paves a way for further studies in animals.关键词
铜绿假单胞菌/外膜蛋白F/VP22/DNA疫苗Key words
Pseudomonas aerufinosa/OprF/VP22/DNA vaccine分类
医药卫生引用本文复制引用
杨勤,雍熙,李婷婷,余娴,雷军..铜绿假单胞菌外膜蛋白F与HSV-1VP22融合DNA疫苗的构建与表达[J].川北医学院学报,2018,33(3):338-342,5.基金项目
国家自然科学基金(81102288) （81102288）
川北医学院校级科研发展计划项目(CBY17-A-YB46) （CBY17-A-YB46）
