动物医学进展2018，Vol.39Issue(6)：24-28,5.
仔猪大肠杆菌病、C型产气荚膜梭菌病二联灭活疫苗的研制与应用
Development and Application of Bivalent Inactivated Vaccine against E.coli and C-type Clostridium perfringens in Piglets
摘要
Abstract
In order to develop bivalent inactivated vaccine against E.coli and C-type Clostridium perfrin-gens in piglets ,the strains C83549(K88) ,C83644(K99) ,C83710(987P) of E.coli and strains C59-2 of C-type Clostridium perfringens were inoculated into suitable media ,respectively .The pili of E.coli and exo-toxin of Clostridium perfringens type C were extracted ,inactivated and detoxificated ,then lyoprotectant was added ,and freeze-dried bivalent inactivated vaccine was made .The vaccine trait test and sterility test were qualified .The vaccine was safe in piglets and pregnant sows .After vaccinating pregnant sows by this vaccine ,the results showed more than 80% protection rate for piglets from E.coli and C-type Clostridium perfringens challenges .The clinical application showed that the vaccine could obviously improve the litter survival in immunized sows .关键词
大肠埃希菌/C型产气荚膜梭菌/二联灭活疫苗/研制与应用Key words
Escherichia coli/C-type Clostridium perfringens/bivalent inactivated vaccine/development and application分类
农业科技引用本文复制引用
刘泽文,袁芳艳,刘威,周丹娜,段正赢,郭锐,杨克礼,田永祥,阮征..仔猪大肠杆菌病、C型产气荚膜梭菌病二联灭活疫苗的研制与应用[J].动物医学进展,2018,39(6):24-28,5.基金项目
湖北省农业科技创新中心资助项目(2016-620-000-001-026) （2016-620-000-001-026）
湖北省重点基金项目(2014CFA106) （2014CFA106）
湖北省科技支撑计划(2014BBB010) （2014BBB010）
湖北省技术创新专项重大项目(2016ABA124) （2016ABA124）
