动物医学进展2018，Vol.39Issue(7)：18-22,5.
H9N2亚型禽流感病毒糖基化位点突变株的构建与生物学特性研究
Construction of N-glycosylation Site Mutant Strains of H9N2 Subtype Avian Influenza Virus and Their Biological Characteristics
摘要
Abstract
To understand the function of H9N2 avian influenza virus (AIV)200 HA protein N-glycosylation site,eight plasmid reverse genetics system were used to rescue the recombinant virus strains r-WZP (loss the 200 N-glycosylation sites of HA gene)and r-WZP200 (gain the 200 N-glycosylation sites of HA gene). All internal genes of the two recombinant virus strains were from A／Puerto Rico／8／34 (H1N1)strain, while the HA and NA genes were from WZP.The two rescued recombinant viruses could propagate stably in SPF chicken embryos and the HA gene has no further mutations.The present studies indicated that loss of the 200 N-glycosylation site could significantly decrease the ability of binding chicken red blood cells,im-prove the thermal stability of HA titers,and enhance their lethal capacity to SPF chicken embryos.This study,for the further study of H9N2 subtype AIV HA protein N-glycosylation sites function,has impor-tant reference value.关键词
H9N2亚型禽流感病毒/HA基因/N-糖基化位点Key words
Avain infuenza virus H9N2 subtype/HA gene/N-glycosylation site分类
农业科技引用本文复制引用
廖昌韬,曾凡桂,严专强,覃健萍,曹永长,谢青梅,陈峰..H9N2亚型禽流感病毒糖基化位点突变株的构建与生物学特性研究[J].动物医学进展,2018,39(7):18-22,5.基金项目
广东省产学研项目(2010B090301019) （2010B090301019）
