动物医学进展2018，Vol.39Issue(7)：47-51,5.
新疆昭苏县马感染嗜吞噬细胞无浆体血清学与分子生物学调查
Serological and Molecular Biological Investigation on Anaplasma phagocytophilum Infection of Horses in Zhaosu County of Xinj iang
摘要
Abstract
Horse is an important natural host of Anaplasma phagocytophilum .The aim of this study was to investi-gate A.phagocytophilum infection and characterize the pathogen in horses in Zhaosu county of Xinj iang province. In this study,an indirect fluorescent antibody assay (IFA)was used to detect A.phagocytophilum antibodies,and the corresponding DNA samples of the antibody positive samples were subjected to PCR detection and sequencing on the basis of 16S rRNA gene (641 bp).The obtained sequences were then used for genetic identification of the pathogen.The serological positive rate of A.phagocytophilum infection was 8%(8／100).The PCR detection fur-ther confirmed the infection of A.phagocytophilum in horses in the investigated regions.Genetic identification found two epidemic strains,which were highly similar to the ones previously reported in China or in its neighboring countries.These data will be helpful for epidemiological investigation or making control measures of the A.phago-cytophilum infection in the surveyed region.关键词
嗜吞噬细胞无浆体/间接免疫荧光/16S rRNA/分子分型Key words
Anaplasma phagocytophilum/indirect fluorescent antibody assay (IFA)/16S rRNA gene/ge-netic identification分类
农业科技引用本文复制引用
李烛南,卢怡竹,王振国,刘志杰,殷宏,曾巧英..新疆昭苏县马感染嗜吞噬细胞无浆体血清学与分子生物学调查[J].动物医学进展,2018,39(7):47-51,5.基金项目
国家自然科学基金项目(31502091) （31502091）
农业科技创新工程项目(ASTIP2014ZL010) （ASTIP2014ZL010）
国家肉牛牦牛产业技术体系项目(NBCISCARS-38) （NBCISCARS-38）
国家重点研发计划项目(2017YFD0501200) （2017YFD0501200）
江苏高校动物重要疫病与人兽共患病防控协同创新中心项目 （）
