中国耳鼻咽喉头颈外科2018，Vol.25Issue(2)：71-74,4.DOI:10.16066/j.1672-7002.2018.02.004
蛇床子素对人鼻咽癌细胞CNE2细胞增殖和凋亡的影响
Effect of Osthole on cell proliferation and apoptosis in human nasopharyngeal carcinoma cell CNE2
摘要
Abstract
OBJECTIVE To study the effect of Osthole on the proliferation and apoptosis in human nasopharyngeal carcinoma(NPC) cell, and to explore new treatment measures for nasopharyngeal carcinoma. METHODS Human NPC cell line CNE2 was treated with various concentrations of Osthole. MTT assay was used to investigate the cell viability, and apoptosis was detected by flow cytometry in CNE2 cells. Furthermore, the mRNA and protein expression levels of Bcl-2, Bax were determined by RT-PCR and western blot respectively. RESULTS Osthole induced significantly inhibitory effect on CNE2 cells at 24 h, 48 h and 72 h, and it was related with time and dose(P＜0.01). Following treatment of Osthole for 48 h, CNE2 cells showed significantly higher apoptosis rate than controls(P＜0.01). Meanwhile, both the mRNA and protein levels of Bax in Osthole-treated CNE2 cells were significantly higher than that of controls, while the level of Bcl-2 was downregulated, both of which changed with dose(P＜0.01). CONCLUSION The present study implied that Osthole can effectively inhibit proliferation and induce apoptosis in NPC cell lines CNE2.关键词
鼻咽肿瘤/蛇床子素/细胞增殖/细胞凋亡Key words
Nasopharyngeal Neoplasms/Osthole/Cell Proliferation/Apoptosis引用本文复制引用
陈炯玉,黄裔腾,庄珊珊,彭琳..蛇床子素对人鼻咽癌细胞CNE2细胞增殖和凋亡的影响[J].中国耳鼻咽喉头颈外科,2018,25(2):71-74,4.基金项目
国家自然科学基金青年基金(81602886)、广东省自然科学基金(2016A030313062)和汕头市科技计划项目([2015]113)联合资助 （81602886）
