福建农业学报2018，Vol.33Issue(5)：451-455,5.DOI:10.19303/j.issn.1008-0384.2018.05.001
禽多杀性巴氏杆菌外膜蛋白H基因的克隆表达及抗原性鉴定
Cloning ,Expression and Antigenicity of Omp H Gene of Pasteurella multocida
摘要
Abstract
The immunological activity of the outer membrane protein H (Omp H) of Pasteurella multocida (Pm) wasidentified.BasedonthepublisheddataonP.multocidastrainX-73(U50907.1),twopairsofprimerswere used to amplify the Omp H of P .multocida strain CVCC 44801 by PCR . The amplified fragment was determined to be 1 348 bp (ORF at 1 056 bp) .The sequence alignment on Omp H of Pm70 ,C44-1 ,P52 ,XJ121 ,X-73 and P-1059 with those registered in GenBank showed a nucleotide homology between 80.2% and 98.4% and an amino acid homology between 79.6% and 98.5% .The recombinant plasmid pET-Omp H was constructed and transformed into BL21. The expressed protein was about 56 ku ,which was close to the expected molecular weight .The western blot also confirmed its immunological activity .The results would provide the information for the establishment of a viable serological detection method and an applicable Omp vaccine against P .multocida .关键词
禽多杀性巴氏杆菌/外膜蛋白H基因/原核表达Key words
Pasteurella multocida/Omp H gene/prokaryotic expression分类
农业科技引用本文复制引用
陈翠腾,程龙飞,刘荣昌,万春和,傅光华,陈珍,朱春华,黄瑜..禽多杀性巴氏杆菌外膜蛋白H基因的克隆表达及抗原性鉴定[J].福建农业学报,2018,33(5):451-455,5.基金项目
福建省科技计划项目——省属公益类科研院所基本科研专项(2015R1023-9 、 2016R1022-9 、 2017R1023-6) （2015R1023-9 、 2016R1022-9 、 2017R1023-6）
国家现代农业产业技术体系建设专项(CARS-42) （CARS-42）
福建省自然科学基金项目(2017J01059 、 2015J01113) （2017J01059 、 2015J01113）
福建省财政专项(FJFS-2017) （FJFS-2017）
