福建农业学报2018，Vol.33Issue(5)：456-462,7.DOI:10.19303/j.issn.1008-0384.2018.05.002
2种多不饱和脂肪酸对樱桃谷鸭脂质和SREBP1基因表达的效应
Effects of Two Polyunsaturated Fatty Acids on Lipid Contents and SREBP1 Expression in Meat-type Ducks
摘要
Abstract
Polyunsaturated fatty acids (PUFAs) play a critical role in animal lipid homeostasis by regulating the expressions of lipogenic and lipolytic genes . This study attempted to decipher the effects of linoleic acid and eicosapentaenoic acid (EPA) in diet on the lipids and the sterol regulatory element binding protein 1 (SREBP1) gene in meat-type ducks .Indoor-raised female Cherry Valley ducks were randomly divided into three groups to be fed each with either a basal diet ,the basal diet+4% linoleic acid ,or the basal diet+4% EPA .Content of lipids and abundance of SREBP1 mRNA were determined in 4 ,6 ,8 and 10 weeks after the feeding started .The addition of linoleic acid and EPA in the diet was found to raise the contents of saturated fatty acids ,PUFAs and essential fatty acids (EFA) in the duck muscles as well as those of triglyceride (TG) ,total cholesterol (TCHO) and high-density lipoprotein (HDL) in the blood .The effect induced by the addition of linoleic acid was greater than that of EPA . The mRNA in liver SREBP1 declined with the added fatty acids suggesting their inhibitory effect on the transcription of the gene ,with a slightly greater extent by EPA than linoleic acid .The expression of SREBP1 significantly correlated with the abdominal fat ,intramuscular fat ,unsaturated fatty acids ,PUFA ,EFA ,serum TG and TCHO in the ducks .It appeared that linoleic acid ,EPA and SREBP1 gene regulation interacted to affect the lipid deposit in the ducks .关键词
肉鸭/亚油酸/二十碳五烯酸/脂质/SREBP1基因Key words
meat-type duck/linoleic acid/eicosapentaenoic acid/lipids/SREBP1 gene分类
农业科技引用本文复制引用
罗华伦,李万贵,张依裕,覃媛钰,吴磊,余蓉蓉..2种多不饱和脂肪酸对樱桃谷鸭脂质和SREBP1基因表达的效应[J].福建农业学报,2018,33(5):456-462,7.基金项目
国家自然科学基金项目(31160453 、 31760663) （31160453 、 31760663）
