福建农业学报2018，Vol.33Issue(5)：474-480,7.DOI:10.19303/j.issn.1008-0384.2018.05.005
基于转录组测序的紫色秋葵花青素合成相关基因研究
Utilizing Transcriptome of Purple Abelmoschus esculentus for Sequence Analysis on Anthocyanidin Gene
摘要
Abstract
The rich content of anthocyanidin in purple Abelmoschus esculentus renders the characteristic color of the plant and its fruits . This study used the transcriptome of the plant to study the genetics associated with the anthocyanidin biosynthesis pathway under natural conditions .The transcriptome was sequenced using the Illumina HiSeq 2500 platform to isolate 35 anthocyanidin unigenes from the Nr , SwissProt and GO databases . These unigenes belonged to 4 distinct gene types including 19 of anthocyanidin-related glucosyltransferases , 6 of anthocyanidin-related acyltransferases , 5 of leucoanthocyanidin dioxygenases , and 5 of anthocyanidin-related reductases .The genes were annotated by Nr and SwissProt databases to show their associations with a variety of plants ,such as Arabidopsis thaliana , Theobroma cacao , Gossypium arboretum , Manihot esculenta , Malus domestica and Vitis vinifera .A clustering analysis indicated that the unigenes had the closest relationship with the leucoanthocyanidin dioxygenase family ,followed by the anthocyanin-related acyltransferases and the reductases , while the anthocyanidin-related glucosyltransferases showed the farthest homology . Identification of the 35 anthocyanidin-related unigenes would be useful for the gene cloning and analysis in studying anthocyanin biosynthesis of purple A .esculentus or other plants rich in anthocyanidin .关键词
紫色秋葵/花青素基因/转录组/测序分析/功能注释Key words
purple Abelmoschus esculentus/anthocyanidin gene/transcriptome/sequencing analysis/function annotation分类
农业科技引用本文复制引用
张少平,应璐,邱珊莲,张帅,洪建基,郑开斌,余文权..基于转录组测序的紫色秋葵花青素合成相关基因研究[J].福建农业学报,2018,33(5):474-480,7.基金项目
福建省科技计划项目——省属公益类科研院所基本科研专项(2016R1012-4) （2016R1012-4）
福建省财政专项——福建省农业科学院青年创新团队建设项目(STIT2017-3-4) （STIT2017-3-4）
福建省农业科学院科研项目(AG2017-5) （AG2017-5）
