福建农业学报2018,Vol.33Issue(5):534-537,4.DOI:10.19303/j.issn.1008-0384.2018.05.017
人工培养和野生铅绿褶菇抗氧化活性研究
Studies on Antioxidant Activity of Artificial Culture and Wild Chlorophyllum molybdites
摘要
Abstract
Lifespan of Caenorhabditis elegans in vivo ,along with the DPPH radical scavenging test in vitro ,was used to evaluate the anti-oxidative activity of Chlorophyllum molybdites .The results showed that the C .molybdites fermentation broth increased the resistance of the nematodes to oxidative stress .At 0.5 mg · mL -1,the broth extended the mean lifespan of the paraquat-treated C .elegans 70% longer as compared to that of CK .The anti-oxidative activity of the water extract of immature fruitbodies was greater than that of the mature fruitbodies of C . molybdites .When treated with the water extract of immature mushrooms at 0.5 and 1.0 mg · mL -1,the mean lifespans of the nematodes were 4.85 d and 5.06 d ,respectively ,which were significantly longer than that of CK . The DPPH scavenging rate of the fermentation broth was the highest ,at 98.14%,followed by that of the water extract of mature fruitbodies ,at 92.86%,and that of the water extract of immature fruitbodies ,at 85.71% .The findings suggested that C . molybdites could potentially be a new source for producing antioxidant from a biomaterial .关键词
铅绿褶菇/抗氧化/DPPH·/秀丽线虫Key words
Chlorophyllum molybdites/antioxidant/DPPH/Caenorhabditis elegans分类
生物科学引用本文复制引用
林群英,龙良鲲,向准,康超,邓春英..人工培养和野生铅绿褶菇抗氧化活性研究[J].福建农业学报,2018,33(5):534-537,4.基金项目
贵州省科学技术基金(黔科合J字[2015] 2057号) (黔科合J字[2015] 2057号)
贵州省科技计划项目(黔科合支撑[2017] 2513) (黔科合支撑[2017] 2513)