分析测试学报2018，Vol.37Issue(6)：669-675,7.DOI:10.3969/j.issn.1004-4957.2018.06.005
超高效液相色谱-串联质谱法测定化妆品中6种生物碱
Determination of 6 Alkaloids in Cosmetics by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry
摘要
Abstract
An ultra performance liquid chromatography-tandem mass spectrometric (UPLC-MS/ MS) method was developed for the determination of six alkaloids, e. g. colchicine, raubasine, ve-ratridine, cevadine, ergotamine and ergocristine in cosmetics. The samples were ultrasonically extracted with a mixed solvent of 0.1％ formic acid-acetonitrile, then cleaned up with a Cleanert PCX solid phase extraction column. After separated on a Thermo Hypersil GOLD column, the analytes were detected in multiple reaction monitoring (MRM) mode. Six alkaloids showed good linearities (r2 >0.999 0) in the range of 0.2-20 μg/L. The limits of quantitation (LOQs) of the method for six alkaloids were in the range of 2.0-5.3 μg/kg. The average recoveries for six alkaloids at three spiked levels ranged from 83.9％ to 101.1％ with relative standard deviations of 0.7％-6. 6％. The method was rapid, accurate and sensitive, and could be applied in the safety evaluation on alkaloids in cosmetics.关键词
超高效液相色谱-串联质谱/化妆品/生物碱/固相萃取Key words
ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/ MS)/cosmetics/alkaloids/solid phase extraction(SPE)分类
化学化工引用本文复制引用
汪晨霞,张瑞瑞,寻知庆,郭新东,王强,黄金凤..超高效液相色谱-串联质谱法测定化妆品中6种生物碱[J].分析测试学报,2018,37(6):669-675,7.基金项目
国家质量监督检验检疫总局科技计划项目(2016QK038) （2016QK038）
