分析测试学报2018，Vol.37Issue(6)：689-695,7.DOI:10.3969/j.issn.1004-4957.2018.06.008
基于分子发夹/荧光微球的侧向层析法定量检测黄曲霉毒素B1
Determination of Aflatoxin B1 by Lateral Flow Assay Based on Molecule Beacon/Fluorescent Microsphere
摘要
Abstract
A new method was proposed for the determination of aflatoxin Bl (AFB1) by lateral flow assay based on molecule beacon (MB)/fluorescent microsphere (FM). A MB containing AFB1 aptamer conjugated with FM, which was coated on the conjugate pad of a strip. A sequence containing adenine(A) was coated on the nitrocellulose membrane (NCM) at the test line, and another sequence complementing with AFB1 aptamer was coated on the control line. In the presence of AFB1, the specific combination of AFB1 and aptamer-FM led to the broken off of the double strand in MB. Then, the complex of AFB1/aptamer-FM was captured at the test line, and the excess MB/FM were captured at the control line. The fluorescence was observed with a fluorescence detector, and the content of AFB1 was estimated by the ratio of fluorescence peaks of the test line (T) and the control line(C). The results showed that there existed a good linear relationship between the ratio of fluorescence peaks and AFB1 in the range of 0.1-50 μg/L with a limit of detection (S/N=3) approximately 0.05 μg/L. With a light signal "off-on" procedure, this proposed method could provide a new way for the accurate determination of AFB1.关键词
黄曲霉毒素B1/分子发夹/荧光微球/侧向层析法/定量分析Key words
aflatoxin B1/molecule beacon(MB)/fluorescent microsphere(FM)/lateral flow assay/quantitative analysis分类
化学化工引用本文复制引用
周子明,谢彩玲,赵斌,郭狄..基于分子发夹/荧光微球的侧向层析法定量检测黄曲霉毒素B1[J].分析测试学报,2018,37(6):689-695,7.基金项目
广东省科技计划项目(2016A020210007) （2016A020210007）
