分析化学2018，Vol.46Issue(7)：1095-1101,7.DOI:10.11895/j.issn.0253-3820.181142
基于DNA杂交链式反应和杂交空位的无标记荧光检测DNA研究
Label-free Fluorescent DNA Detection Strategy Based on Hybridization Chain Reaction and Vacant Site-Binding Molecule
摘要
Abstract
A label-free and enzyme-free fluorescent DNA sensor based on hybridization chain reaction ( HCR) and vacant site-binding molecule was developed. By rationally incorporating a cytosine in hairpin DNAs for HCR and utilizing vacant site-binding fluorophore ATMND, sensitive DNA detection was achieved, resulting from the large amount of vacant sites presented on the long DNA nanostructures upon HCR. Gel electrophoresis and atomic force microscopy were performed to characterize the hybridization chain reaction. Under the optimized conditions, the method could sensitively detect the target DNA with the low detection limit of 2. 0 nmol/L. The method shows great potential in signal amplification for DNA detection with advantages such as simple, label-free and low-cost.关键词
杂交链式反应/杂交空位/荧光/信号放大/无标记Key words
Hybridization chain reaction/Vacant site/Fluorescence/Signal amplification/Label-free引用本文复制引用
徐杰,林滨,王亚,徐志爱,程桂英,张文..基于DNA杂交链式反应和杂交空位的无标记荧光检测DNA研究[J].分析化学,2018,46(7):1095-1101,7.基金项目
本文系国家自然科学基金项目(Nos. 21775046, 21675055)资助 This work was supported by the National Natural Science Foundation of China (Nos. 21775046, 21675055). （Nos. 21775046, 21675055）
