分析化学2018，Vol.46Issue(7)：1152-1162,11.DOI:10.11895/j.issn.0253-3820.171394
梅花鹿茸快速生长及骨化期相关差异表达蛋白分析
Analysis of Differential Proteins of Sika Deer Antler at Rapid Growth and Ossification Stages
摘要
Abstract
Differential proteomics analysis of Sika deer antlers at rapid growth stage (60 d) and ossification stage ( 90 d) was performed by isobaric tags for relative and absolute quantitation ( iTRAQ ) , ultra high performance liquid chromatography and mass spectrometry technologies. A total of 127 differential proteins were identified. Compared with the ossification stage, 80 differential proteins were significantly up-regulated and 47 differential proteins were significantly down-regulated at the rapid growth stage. These differential proteins were mainly distributed in the regions of extracellular matrix, nucleosome, haptoglobin-hemoglobin complex, actin filament, endoplasmic reticulum-Golgi intermediate compartment, endoplasmic reticulum lumen, and endometrium, etc. The up-regulated differential proteins were mainly involved in the regulations of oxygen transport in the blood, nerve growth and regeneration, cartilage and bone development and ATP synthesis compared with ossification stage, and the down-regulated differential proteins were mainly involved in the endochondral ossification process. The changes of protein expression at different growth stages were closely related to antler rapid growth and ossification. Therefore, the results of this study provided a basic data for discovering the molecular mechanisms of antler rapid growth and ossification, and it was of great significance for further study of the pharmacological basis and clinical application of antlers.关键词
梅花鹿茸/快速生长期/骨化期/同位素标记相对定量技术/蛋白质组学/差异蛋白Key words
Sika deer antler/Rapid growth stage/Ossification stage/Isobaric tags for relative quantitation/Proteomics/Differential proteins引用本文复制引用
幺宝金,张梅,刘美辰,王群,刘宇昕,赵雨..梅花鹿茸快速生长及骨化期相关差异表达蛋白分析[J].分析化学,2018,46(7):1152-1162,11.基金项目
本文系吉林省教育厅"十三五"科学技术研究项目( No. JJKH20170721KJ )和吉林省科技厅优秀青年人才基金项目(No. 20170520044JH)资助 This work was supported by the Science and Technology Project of Jilin Provincial Education Department ( No. JJKH20170721KJ) and the Science and Technology Development Project of Jilin Province, China (No. 20170520044JH) . （ No. JJKH20170721KJ ）
