高等学校化学学报2018，Vol.39Issue(7)：1580-1586,7.DOI:10.7503/cjcu20170749
HA/GCPU复合多孔支架的可控制备及细胞相容性
Preparation and Cytocompatibility of Hydroxyapatite/glyceride Based Polyurethane Porous Scaffolds
摘要
Abstract
Castor oil was transesterified with glycerol,the glyceride of castor oil(GCO)copolymerized with isophorone diisocyanate(IPDI)to generate polyurethane(PU).The porous scaffolds of hydroxyapatite/glyceride based polyurethae(HA/GCPU)were prepared by in situ polymerization.The GCPU and HA/GCPU porous scaffolds were characterized by Fourier transforw infrared spectroscopy(FTIR)and scanning electron microscopy(SEM).The mechanical property and porosity were tested.The results showed that the porosity of GCPU,HA/GCPU composite with 20% HA and HA/GCPU composite with 40% HA were(61 ± 3)%,(68±2)% and(57 ± 3)%,respectively.The compressive strength of above three porous scaffolds were 605±61,2125±58 and(4588±260)kPa,respectively.To further evaluate the cytocompatibility of porous scaffolds,the MG63 cells were co-cultured on scaffolds,and light microscopy observation,SEM observation,3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium-bromide(MTT)assay were carried out.The results demonstrated that both pure GCPU and HA/GCPU scaffolds had no cytotoxicity.It is believed that HA/GCPU composite porous scaffolds have the potential to be applied in bone regeneration.关键词
蓖麻油/甘油酯聚氨酯/羟基磷灰石/多孔支架/细胞相容性Key words
Castor oil/Glyceride polyurethane/Hydroxyapatite/Porous scaffolds/Cytocompatibility分类
化学化工引用本文复制引用
杜晶晶,黄棣,魏延,连小洁,胡银春,王楷群,刘阳,陈维毅..HA/GCPU复合多孔支架的可控制备及细胞相容性[J].高等学校化学学报,2018,39(7):1580-1586,7.基金项目
国家自然科学基金(批准号:11502158,51503140,11632013,11502156)和山西省青年科技基金(批准号:2015021195)资助.Supported by the National Natural Science Foundation of China(Nos.11502158,51503140,11632013,11502156)and the Natural Science Foundation of Shanxi Province,China(No.2015021195). （批准号:11502158,51503140,11632013,11502156）
