广东医学2018，Vol.39Issue(3)：336-339,4.
PAO1及其突变菌株对诱导巨噬细胞产生抗菌肽LL-37的作用
Study on the correlation of PAO1 and its mutant strain on the production of antimicrobial peptide LL-37 in macrophages
摘要
Abstract
Objective To study the effects of PAO1 and its mutant strains of Pseudomonas aeruginosa ( PA) on the production of antimicrobial peptide LL-37 in macrophages. Methods Immunomagnetic beads method was applied for isolation of human peripheral blood CD14 + monocytes. Recombinant human granulocyte monocyte set colony stimulating factor was used for the induced-differentiation of macrophages. Different concentrations of PAO1, PA-△lasI, PA-△rhlI and PA-△lasI /rhlI were used to process the macrophages. The enzyme linked immunosorbent assay ( ELISA) method was used for the assessment of antimicrobial peptide LL-37 concentrations produced by PA-induced macrophage. Results Different concentrations of PAO1-, PA-△lasI-, PA-△rhlI-and PA-△lasI /rhlI-induced macrophages began to secrete antimicrobial peptides at 12 h, reached peak at 24 h or 48 h, and gradually declined until disappeared at 72 h, in dose-time-dependent manners. LL-37 was highest in the PA-ΔlasI / rhlI: macrophages =100∶ 1( 1. 631 2 ± 0. 277 0) ng /mL. Conclusion The antimicrobial peptide LL-37 is induced in time-dependent and concentration-dependent manners. Double mutant strain is more conducive for macrophages to produce LL-37, helping PA escape the host immune defense.关键词
铜绿假单胞菌/群体感应系统/巨噬细胞/抗菌肽LL-37Key words
pseudomonas aeruginosa/quorum sensing system/macrophage/antimicrobial peptide LL-37引用本文复制引用
肖倩,赵丹,张云燕,曾建明,李有强,陈茶,张伟铮,蔡壬辛..PAO1及其突变菌株对诱导巨噬细胞产生抗菌肽LL-37的作用[J].广东医学,2018,39(3):336-339,4.基金项目
广东省医学科学技术研究基金项目(编号:A2015457),广东省中医药局科研项目(编号:20172075),广东省中医院中医药科学技术研究专项资助项目(编号:YN2016QJ13) （编号:A2015457）
